Autoimmune Antibodies

ABSTRACT

The invention generally relates to biomarkers associated with NSCLC, and methods and compositions for the detection and diagnosis of non-small cell lung cancer in a human subject.

RELATED APPLICATIONS

This application claims the benefit of EP Application No. 12171126.1,filed Jun. 7, 2012. All the teachings of the above-referencedapplication is incorporated herein by reference.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has beensubmitted in ASCII format via EFS-Web and is hereby incorporated byreference in its entirety. Said ASCII copy, created on Jun. 3, 2013, isnamed P4928SeqList.txt and is 128,129 bytes in size.

FIELD OF THE INVENTION

The present invention provides methods for identifying patientsdiagnosed with non-small cell lung cancer who will most benefit fromtreatment with erlotinib comprising detecting autoantibodies in bloodserum of said patients.

BACKGROUND OF THE INVENTION

Tarceva® is an orally active, potent, inhibitor of the epidermal growthfactor receptor (EGFR) tyrosine kinase (TKI).

Erlotinib hydrochloride is the active ingredient in Tarceva®, which isapproved as single agent treatment for patients with advanced non-smallcell lung cancer (NSCLC) after treatment with chemotherapy, asmaintenance treatment for patients not progressing during chemotherapy(1^(st) line maintenance) or after failure of chemotherapy(2^(nd)/3^(rd) line maintenance). Tarceva® is also approved as firstline treatment for patients whose tumor harbors an EGFR activatingmutation in the EU.

Expression of unusual proteins is common in cancer and viral infection.The mammalian immune system contains a specialized arm that recognizesproteins induced by cellular transformation (neo-antigens) andeffectively eliminates cells that express these neo-antigens againstwhich tolerance has not established during development. This arm of theimmune system is thought to be effective against viral infections,spontaneous chromosomal and genomic rearrangements caused by errors ofthe cell division machinery, or carcinogen-induced transformingmutations. While the initial cytotoxic immune response—which is mediatedby the recognition of the unusual proteins displayed on MHCI complexesby CD8⁺ T-cells—is fast and effective, a sustained response againstvirus infections or aberrant cellular clones requires the co-stimulatoryeffect of CD4⁺ T-helper cells which are activated after presentation ofextracellular peptides (which can stem from cells lysed in the firstphase of the cytotoxic immune response) via MHCII complexes ofprofessional antigen presenting cells. Additionally, these cells areable to induce a lasting B-cell response and antibodies to viral oraberrant proteins. The infiltration of a tumor with large numbers ofCD8⁺ T-cells has shown to be a better prognostic marker than traditionaltumor staging in colorectal cancer (Koizumi, Hojo et al. 2007¹), and ithas been associated with favorable prognosis in almost all major solidcarcinomas independently of cancer cell type. ¹Koizumi, K., S. Hojo, etal. (2007). “Chemokine receptors in cancer metastasis and cancercell-derived chemokines in host immune response.” Cancer Science 98(11):1652-1658

Autoantibodies are well known diagnostic entities in cancer. Many cancerautoantibodies are proteins that are normally expressed in embryonictissues and therefore represent a “foreign” protein against which immunetolerance is not established. The shedding of these proteins to thebloodstream late in cancer history leads to a humoral immune response. Atypical example is the common cancer marker CEA (cancer embryonicantigen). Tumors frequently exhibit faulty protein processing. Prominentexamples include proteins that are incorrectly cleaved by celllocalization proteases (the presence of autoantibodies against theN-terminal sequence of p53 which is normally cleaved after the proteinis targeted to the nucleus is one of the most specific biomarkers oflung cancer) or incorrectly glycosylated by cellular glycosylases(anti-MUC1-antibodies against a form of MUC1 that is incorrectlyO-glycosylated is a biomarker for a variety of cancers). Even though p53mutations are frequently observed in tumors, the specificity of theautoantibodies against this protein is almost always against theN-terminus of the protein—connecting the immunogenicity of p53 more tothe ectopic expression of a protein that is normally short lived andlocated in the nucleus.

The detection of natural EGFR autoantibodies in serum of NSCLC patientshas been described by Li et al.². Li et al.³ and Chapman et al.⁴described the detection of natural autoantibodies to p53, HER2,NY-ESO-1, CAGE, MUC1 and GBU4-5. Further, WO2011073905⁵ relates to tumormarkers associated with the progression of a cancer disease from a lessprogressed stage to a more progressed stage. ²Li Yuan et al, ChineseJournal of Lung Cancer, 13(7), 2010, 727-730³Li Yuan et al, ChineseJournal of Lung Cancer, 12(10), 2009, 1999-6187⁴C J Chapman, A Murray, JE McElveen, U Sahin, U Luxemburger, Ö Türeci, R Wiewrodt, A C Barnes, JF Robertson, “Autoantibodies in lung cancer: possibilities for earlydetection and subsequent cure”, Thorax 2008; 63(3):228-233⁵WO2011073905

Unusual (mutated or expressed in abnormal quantities or locations) tumorproteins can invoke an antibody response. Tumor-induced autoantibodieshave frequently been observed and their utility as diagnostic markershas been investigated (Albert and Darnell 2004⁶). While autoantibodiesto tumor proteins can be found that have exquisite specificity, most ofthem lack the required sensitivity for a diagnostic test. ⁶Albert, M. L.and R. B. Darnell (2004). “Paraneoplastic neurological degenerations:Keys to tumor immunity.” Nat. Rev. Cancer 4(1): 36-44

Few reliable epidemiologic and genetic markers that predict erlotinibhydrochloride response are known in the art. In particular, EGFRmutations in exon 18-21 (intracellular kinase domain of the receptor)were described to be linked with better prognosis as well as with betterresponse to TKI treatment (Paz-Ares, Soulieres et al. 2010⁷). The onlypredictive marker currently known (EGFR activating mutation) isdifficult to diagnose as a biopsy of the tumor is needed. At presentonly about 50% of NSCLC patients are diagnosed with a biopsy (Reck,Hermes et al. 2011⁸). There is a definite need for simpler techniques todetect predictive markers of TKI efficacy. It is desirable to identifythe responders at diagnosis in order to have as many patients aspossible benefiting from erlotinib treatment as early as possible, whileexploring other treatment options for patients not likely to respond.⁷Paz-Ares, L., D. Soulieres, et al. (2010). “Clinical outcomes innon-small-cell lung cancer patients with EGFR mutations: pooledanalysis.” J Cell Mol Med 14(1-2): 51-69⁸Reck, M., A. Hermes, et al.(2011). “Tissue sampling in lung cancer: A review in light of the MERITexperience.” Lung Cancer 74(1): 1-6

DETAILED DESCRIPTION OF THE INVENTION

Present invention solves that problem in that it provides a method ofdiagnosis of cancer in a human subject.

Present invention solves that problem in that it provides a method ofdiagnosis of non-small cell lung cancer in a human subject.

Present invention solves that problem in that it provides a method ofdiagnosis of non-small cell lung cancer in a human subject by providingantibodies against mutated EGFR sequences.

A patient identified by a method as described herein is a patient, inparticular a NSCLC patient who will respond to the treatment witherlotinib or a pharmaceutically acceptable salt thereof, in particularerlotinib hydrochloride.

We surprisingly found that present autoantibodies possess the requiredsensitivity for a diagnostic test, especially autoantibodies againstmutated EGFR sequences.

EGFR is normally bound to the cell membrane and not shed to thebloodstream. EGFR is a normal adult protein that is found in largequantities in some adult tissues. Immune tolerance is expected to beestablished against this protein and many of its variants. Almost all ofthe sequences belong to the cytoplasmic part of the molecule and areinvisible to professional antigen presenting cells. The mutations affectonly a small part of the EGFR molecule.

Unless defined otherwise, all terms used herein have the same meaningsas commonly understood by a skilled artisan to which art this inventionbelongs.

The term “a level of said autoantibody representative for a humansubject of a healthy population” refers to an estimate of the mean levelof the autoantibody in serum of a population of patients who do notsuffer from NSCLC.

The term “a level of said autoantibody representative for a NSCLCpatient” refers to an estimate of the mean level of the autoantibody inserum of a population of patients who suffer from NSCLC.

The term “overall survival (OS)” refers to the length of time fromdiagnosis of disease during and after treatment the patient survives. Asthe skilled person will appreciate, a patient's overall survival isimproved or enhanced, if the patient belongs to a subgroup of patientsthat has a statistically significant longer mean survival time ascompared to another subgroup of patients.

The term “progression-free survival (PFS)” refers to the length of timefrom diagnosis of disease during and after treatment during which,according to the assessment of the treating physician or investigator,the patient's disease does not become worse, i.e., does not progress. Asthe skilled person will appreciate, a patient's progression-freesurvival is improved or enhanced if the patient belongs to a subgroup ofpatients that has a longer length of time during which the disease doesnot progress as compared to the average or mean progression freesurvival time of a control group of similarly situated patients.

The term “patient” refers to any single mammal for which treatment isdesired. In particular, the “patient” is a human subject. Moreparticularly, the patient is a human subject suffering from cancer, inparticular NSCLC.

The term “autoantibody” is a type of protein manufactured by the immunesystem of a patient that is directed against one or more of thepatient's own proteins.

The term “amino acid” denotes the group of naturally occurring carboxya-amino acids comprising alanine (three letter code: ala, one lettercode: A), arginine (arg, R), asparagine (asn, N), aspartic acid (asp,D), cysteine (cys, C), glutamine (gln, Q), glutamic acid (glu, E),glycine (gly, G), histidine (his, H), isoleucine (ile, I), leucine (leu,L), lysine (lys, K), methionine (met, M), phenylalanine (phe, F),proline (pro, P), serine (ser, S), threonine (thr, T), tryptophan (trp,W), tyrosine (tyr, Y), and valine (val, V).

As used herein, “therapy” or “treatment” (and grammatical variationsthereof such as “treat” or “treating”) refers to clinical interventionin an attempt to alter the natural course of a disease in the individualbeing treated, and can be performed either for prophylaxis or during thecourse of clinical pathology. Desirable effects of treatment include,but are not limited to, preventing occurrence or recurrence of disease,alleviation of symptoms, diminishment of any direct or indirectpathological consequences of the disease, preventing metastasis,decreasing the rate of disease progression, amelioration or palliationof the disease state, and remission or improved prognosis.

The term “gene” as used herein comprises variants of the gene. The term“variant” relates to nucleic acid sequences which are substantiallysimilar to the nucleic acid sequences given by the GenBank accessionnumber. The term “substantially similar” is well understood by a personskilled in the art. In particular, a gene variant may be an allele whichshows nucleotide exchanges compared to the nucleic acid sequence of themost prevalent allele in the human population. Preferably, such asubstantially similar nucleic acid sequence has a sequence similarity tothe most prevalent allele of at least 80%, preferably at least 85%, morepreferably at least 90%, most preferably at least 95%. The term“variants” is also meant to relate to splice variants.

The term “mutation” refers to changes in a genomic sequence. Theserandom sequences can be defined as sudden and spontaneous changes in thecell. Mutation can result in several different types of change insequences; these can either have no effect, alter the product of a gene,or prevent the gene from functioning properly or completely. The term“somatic mutation” refers to a change in the genetic structure that isneither inherited nor passed to offspring.

The phrase “recommending a treatment” as used herein refers to using theinformation or data generated relating to the level or presence of abiomarker or an autoantibody in a sample of a patient to identify thepatient as suitably treated or not suitably treated with a therapy. Insome embodiment the therapy may comprise a drug. The information or datamay be in any form, written, oral or electronic. In some embodiments,using the information or data generated includes communicating,presenting, reporting, storing, sending, transferring, supplying,transmitting, delivering, dispensing, or combinations thereof. In someembodiments, communicating, presenting, reporting, storing, sending,transferring, supplying, transmitting, delivering, dispensing, orcombinations thereof are performed by a computing device, analyzer unitor combination thereof. In some further embodiments, communicating,presenting, reporting, storing, sending, transferring, supplying,transmitting, delivering, dispensing, or combinations thereof areperformed by a laboratory or medical professional. In some embodiments,the information or data includes a comparison of the level of saidbiomarker or autoantibody to a reference level. In some embodiments, theinformation or data includes an indication that said biomarker orautoantibody is present or absent in the sample. In some embodiments,the information or data includes an indication that the patient issuitably treated or not suitably treated with a therapy comprising saiddrug. In some embodiments, the therapy is erlotinib.

The phrase “providing a diagnosis” as used herein refers to using theinformation or data generated relating to the level or presence of abiomarker or an autoantibody in a sample of a patient to diagnose adisease in the patent. The information or data may be in any form,written, oral or electronic. In some embodiments, using the informationor data generated includes presenting, reporting, storing, sending,transferring, supplying, transmitting, delivering, dispensing, orcombinations thereof. In some embodiments, presenting, reporting,storing, sending, transferring, supplying, transmitting, delivering,dispensing, or combinations thereof are performed by a computing device,analyzer unit or combination thereof. In some embodiments, presenting,reporting, storing, sending, transferring, supplying, transmitting,delivering, dispensing, or combinations thereof are performed by alaboratory or medical professional. In some embodiments, the informationor data includes a comparison of the level of said biomarker orautoantibody to a reference level. In some embodiments, the informationor data includes an indication that said biomarker or autoantibody ispresent or absent in the sample. In some embodiments, the information ordata includes an indication that the patient is diagnosed with saiddisease. In some embodiments, said disease is non-small cell lungcancer.

During the TASK study, biopsy material had been collected and the tumorcells have been tested for the presence of the most frequent somaticmutations, i.e. a deletion at exon 19, and a point mutation at exon 21.During the TASK study serum samples had been collected at various timepoints (pre-dose, day 8, day 22 and progression) from all patients andwere assessed for autoantibodies against EGFR.

In these patients, autoantigenic peptide sequences that predictdevelopment of rash, or prolonged progression free or overall survivalinevitably include a set of sequences that are derived from the EGFRsequence starting at position 737 and extending through 756. Thesepeptides include a number of sequence variants, but inevitably includesequences that have a deletion at position 746-750 or close nearby(Table 2).

This corresponds exactly to the position of the deletion in the exon 19somatic mutation, known to be predictive for outstanding efficacy fromtreatment with TKI (Rosell et al. 2009⁹, Mok et al. 2009¹⁰). ⁹Rosell etal., N Engl J Med 2009; 361:958-967¹⁰Mok et al., N Engl J Med 2009;361:947-957

The presence of a somatic EGFR mutation in exon 19 in tumor tissue leadsto protein variant against that the immune system has not developedtolerance. The somatic mutation occurs only in the tumor, and therefore,if it induces an autoantibody which can be detected in the patient'sserum it can be used to draw conclusions about the presence of an exon19 mutation or exon 21 mutation in the NSCLC tissue, which is well knownto predict increased progression free survival and superiority oftyrosine kinase monotherapy over chemotherapy (Heigener and Reck2011¹¹). ¹¹Heigener, D. and M. Reck (2011). “Mutations in the epidermalgrowth factor receptor gene in non-small cell lung cancer: Impact ontreatment beyond gefitinib and erlotinib.” Advances in Therapy 28(2):126-133

The autoantibody can be detected using a standard blood sample from thepatient without the need to obtain tumor cells with a biopsy. This is alarge advantage over the current practice, as recovery rates of usefultumor samples even in clinical studies do not exceed 50% (Reck, Hermeset al. 2011¹²). ¹²Reck, M., A. Hermes, et al. (2011). “Tissue samplingin lung cancer: A review in light of the MERIT experience.” Lung Cancer74(1): 1-6

Anti-EGFR autoantibodies can be detected in blood samples of NSCLCpatients at higher concentrations than in healthy controls (FIG. 1). Inparticular, peptide sequences have been identified that yieldconsecutive regions of high immunogenicity with large differencesbetween patient and healthy controls sera. Consecutive sequencestretches were identified, where ratios of individual peptide signals inmore than 30% of the cancer patients to maximum value in controls waslarger than 8 (Table 1).

TABLE 1  Consecutive EGFR sequences with highautoantigenicity in NLSCL patients Consensus sequence of Sequenceautoantigenic Protein Region peptides in the region EGFR 323-336VRKSKKSEGPSxKV EGFR 546-564 PREFVENSECIQCHPECL EGFR 574-591RGPDNCIQCAHYIDGPHCVKTCP EGFR 735-762 GEKVKIPVAIK[-S]PKANK Del 1 EGFR739-758 KIPVAIK[-HRK]PTSPK Del 2 EGFR 793-806 MPFGCLLDYVREH EGFR 867-883KEYHAEGGKVPIKWM EGFR  986-1002 RMHLPSPTDSNFYRA EGFR 1081-1095SIDDTFLPVPEYIN EGFR 1148-1166 NSTFDSPAHWAQKGSHQI

The above sequences can be used for the early diagnosis of NSCLC.

Regression analysis has identified significant evidence that thepresence of antibodies to the peptides influence both, progression freesurvival (PFS) and overall survival (OS) (FIG. 2). Although the numberof samples is small in comparison to the number of peptides, manycovariates need to be considered and none of the individual peptidesreach sufficient statistical significance, we surprisingly found thatwhen combining overlapping information from various independentstatistical approaches, selection of a relevant peptide from the manycandidates obtained after univariate analysis is possible. FIG. 3illustrates the process that led to selection of the candidate sequenceslisted in Table 2. Sequences listed in Table 2 or any continuoussubsequences thereof longer than 8 amino acids are candidate sequences.

TABLE 2  Antigenic sequences influencing PFS and OS of NSCLC patientsConsensus sequence of Sequence autoantigenic Protein Regionpeptides in the region Source EGFR 737-756 KVKIPVAIKELREATSPKA PFS ~Peptide and EGFR 737-756 Del  KVKIPVAIK------[SA]PKA treatment in EGFR -742-748 mutation positive patients with rash EGFR 763-776A[YV]VMASVDNPHVCR Consensus of EGFR 703-717 LLRILKETE[FS]KKI statisticalEGFR 825-838 MNYLEDR[RL]LVHRD analyses for PFS EGFR 296-309KGNYVVTDHGSCVRA Consensus of EGFR 628-641 CTGPGLEGCPTNG statistical EGFR681-727 RLLQEREL[VL]EPLTPSGEAPNQA[L analyses for OSPF]LR[IT]L[KM]ETE[FL]KK[IL] [KF]VLG[SP]GAFGT EGFR 761-780DEAYVMASVDNPHVCRLLG EGFR 830-843 YLEDRRLVHRDLA

TABLE 3  Examples for peptide sequences with highpredictive potential for prolongedprogression free survival in patients with EGFR mutations that develop rash KVKIPVAIKELREATSPKA AnnotationKVKIPVAIK------APKA 737_V003 KVKIPVAIK---APTS 737_V004KVKIPVAIKD------PKA 737_V007 KVKIPVAIKELKA 737_V015 KVKIPVAIKE------PKA737_V019 KVKIPVAIKEQKA 737_V024 KVKIPVAIKESKA 737_V029KVKIPVAIKEV-----PK 737_V037 KVKIPVAIK------IPKA 737_V039KVKIPVAIK------SPKA 737_V054 KVKIPVAIK------TPKA 737_V057--KIPVAIKE----ASPKA 739 V010 --KIPVAIKEF----SPKA 739_V013--KIPVAIKE----NSPKA 739_V027 --KIPVAIK----VASPKA 739_V067--KIPVAIK----VPSPKA 739_V070

EGFR peptide sequences selected from Seq. Id. No. 1-Seq. Id. No. 15 areconsecutive sequences with high autoantigenicity in NLSCL patients.

EGFR peptide sequences selected from Seq. Id. No. 16-Seq. Id. No. 517are useful for predicting the occurrence and grade of adverse eventslike rash to erlotinib treatment.

EGFR peptide sequences selected from Seq. Id. No. 518-Seq. Id. No. 602are antigenic sequences influencing, in particular extending,progression free and overall survival of NSCLC patients, in particularmutated EGFR peptide sequences Seq. Id. No. 554, Seq. Id. No. 555, Seq.Id. No. 556, Seq. Id. No. 557, Seq. Id. No. 558, Seq. Id. No. 559 andSeq. Id. No. 560, as well as EGFR peptide sequences Seq. Id. No. 519,Seq. Id. No. 520, Seq. Id. No. 521 and Seq. Id. No. 561.

EGFR peptide sequences selected from Seq. Id. No. 603-Seq. Id. No. 619have high predictive potential for prolonged progression free survivalin patients with EGFR mutations that develop rash.

Antibodies against these peptide sequences most likely influence PFS andOS if they are present in patients. Tests that detect the presence ofthese antibodies in patient sera could be used to guide treatment andstratify patients into treatment groups.

Present invention provides a method of diagnosis of non-small cell lungcancer in a human subject comprising:

measuring in a blood sample of the human subject a level of anautoantibody selected from the group of autoantibodies recognizingmutated human EGFR, wherein an increased level of said autoantibodyselected from the group of autoantibodies recognizing mutated human EGFRin the blood sample of the human subject compared to a level of saidautoantibody representative for a human subject of a healthy populationis indicative for non-small cell lung cancer.

Present invention provides a method of diagnosis of non-small cell lungcancer in a human subject comprising:

a) measuring in a blood sample of the human subject a level of anautoantibody selected from the group of autoantibodies recognizingmutated human EGFR,

b) comparing the level of said autoantibody to a reference level, and

c) providing a diagnosis of non-small cell lung cancer when the level ofsaid autoantibody is above the reference level.

Present invention provides a method of diagnosis of non-small cell lungcancer in a human subject comprising:

a) measuring in a blood sample of the human subject a level of anautoantibody selected from the group of autoantibodies recognizingmutated human EGFR,

b) comparing the level of said autoantibody to a reference level, and

c) recommending a treatment when the level of said autoantibody is abovethe reference level.

A certain embodiment of the present invention provides a method asdescribed above, wherein said treatment is erlotinib.

A certain embodiment of present invention provides a method as describedherein, wherein the level of an autoantibody recognizing mutated humanEGFR is measured.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes a mutated EGFR peptide isselected from the group consisting of Seq. Id. No. 1, Seq. Id. No. 2,Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7, Seq. Id.No. 15, Seq. Id. No. 16, Seq. Id. No. 17, Seq. Id. No. 18, Seq. Id. No.19, Seq. Id. No. 20, Seq. Id. No. 21, Seq. Id. No. 22, Seq. Id. No. 23,Seq. Id. No. 24, Seq. Id. No. 25, Seq. Id. No. 26, Seq. Id. No. 27, Seq.Id. No. 28, Seq. Id. No. 29, Seq. Id. No. 30, Seq. Id. No. 31, Seq. Id.No. 32, Seq. Id. No. 33, Seq. Id. No. 34, Seq. Id. No. 35, Seq. Id. No.36, Seq. Id. No. 37, Seq. Id. No. 38, Seq. Id. No. 39, Seq. Id. No. 40,Seq. Id. No. 41, Seq. Id. No. 42, Seq. Id. No. 43, Seq. Id. No. 44, Seq.Id. No. 45, Seq. Id. No. 46, Seq. Id. No. 47, Seq. Id. No. 48, Seq. Id.No. 49, Seq. Id. No. 50, Seq. Id. No. 51, Seq. Id. No. 52, Seq. Id. No.53, Seq. Id. No. 54, Seq. Id. No. 55, Seq. Id. No. 56, Seq. Id. No. 57,Seq. 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Id.No. 457, Seq. Id. No. 458, Seq. Id. No. 459, Seq. Id. No. 460, Seq. Id.No. 461, Seq. Id. No. 462, Seq. Id. No. 463, Seq. Id. No. 464, Seq. Id.No. 465, Seq. Id. No. 466, Seq. Id. No. 467, Seq. Id. No. 468, Seq. Id.No. 469, Seq. Id. No. 470, Seq. Id. No. 471, Seq. Id. No. 472, Seq. Id.No. 473, Seq. Id. No. 474, Seq. Id. No. 475, Seq. Id. No. 476, Seq. Id.No. 477, Seq. Id. No. 478, Seq. Id. No. 479, Seq. Id. No. 480, Seq. Id.No. 481, Seq. Id. No. 482, Seq. Id. No. 483, Seq. Id. No. 484, Seq. Id.No. 485, Seq. Id. No. 486, Seq. Id. No. 487, Seq. Id. No. 488, Seq. Id.No. 489, Seq. Id. No. 490, Seq. Id. No. 491, Seq. Id. No. 492, Seq. Id.No. 493, Seq. Id. No. 494, Seq. Id. No. 495, Seq. Id. No. 496, Seq. Id.No. 497, Seq. Id. No. 498, Seq. Id. No. 499, Seq. Id. No. 500, Seq. Id.No. 501, Seq. Id. No. 502, Seq. Id. No. 503, Seq. Id. No. 504, Seq. Id.No. 505, Seq. Id. No. 506, Seq. Id. No. 507, Seq. Id. No. 508, Seq. Id.No. 509, Seq. Id. No. 510, Seq. Id. No. 511, Seq. Id. No. 512, Seq. Id.No. 513, Seq. Id. No. 514, Seq. Id. No. 515, Seq. Id. No. 516, Seq. Id.No. 517, Seq. Id. No. 518, Seq. Id. No. 522, Seq. Id. No. 523, Seq. Id.No. 524, Seq. Id. No. 526, Seq. Id. No. 527, Seq. Id. No. 528, Seq. Id.No. 529, Seq. Id. No. 530, Seq. Id. No. 531, Seq. Id. No. 532, Seq. Id.No. 533, Seq. Id. No. 534, Seq. Id. No. 535, Seq. Id. No. 536, Seq. Id.No. 537, Seq. Id. No. 538, Seq. Id. No. 539, Seq. Id. No. 540, Seq. Id.No. 541, Seq. Id. No. 542, Seq. Id. No. 543, Seq. Id. No. 544, Seq. Id.No. 545, Seq. Id. No. 546, Seq. Id. No. 547, Seq. Id. No. 548, Seq. Id.No. 549, Seq. Id. No. 550, Seq. Id. No. 551, Seq. Id. No. 552, Seq. Id.No. 553, Seq. Id. No. 554, Seq. Id. No. 555, Seq. Id. No. 557, Seq. Id.No. 559, Seq. Id. No. 560, Seq. Id. No. 562, Seq. Id. No. 563, Seq. Id.No. 564, Seq. Id. No. 565, Seq. Id. No. 567, Seq. Id. No. 568, Seq. Id.No. 569, Seq. Id. No. 570, Seq. Id. No. 571, Seq. Id. No. 572, Seq. Id.No. 573, Seq. Id. No. 574, Seq. Id. No. 575, Seq. Id. No. 576, Seq. Id.No. 577, Seq. Id. No. 578, Seq. Id. No. 579, Seq. Id. No. 580, Seq. Id.No. 581, Seq. Id. No. 582, Seq. Id. No. 583, Seq. Id. No. 584, Seq. Id.No. 585, Seq. Id. No. 586, Seq. Id. No. 587, Seq. Id. No. 588, Seq. Id.No. 589, Seq. Id. No. 590, Seq. Id. No. 591, Seq. Id. No. 592, Seq. Id.No. 593, Seq. Id. No. 594, Seq. Id. No. 595, Seq. Id. No. 596, Seq. Id.No. 597, Seq. Id. No. 598, Seq. Id. No. 599, Seq. Id. No. 601, Seq. Id.No. 604, Seq. Id. No. 605, Seq. Id. No. 606, Seq. Id. No. 607, Seq. Id.No. 608, Seq. Id. No. 609, Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id.No. 612, Seq. Id. No. 613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id.No. 616, Seq. Id. No. 617, Seq. Id. No. 618 and Seq. Id. No. 619.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the groups consisting of Seq. Id. No. 1, Seq. Id. No.2, Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7 andSeq. Id. No. 15.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the group consisting of Seq. Id. No. 16, Seq. Id. No.17, Seq. Id. No. 18, Seq. Id. No. 19, Seq. Id. No. 20, Seq. Id. No. 21,Seq. Id. No. 22, Seq. Id. No. 23, Seq. Id. No. 24, Seq. Id. No. 25, Seq.Id. No. 26, Seq. Id. No. 27, Seq. Id. No. 28, Seq. Id. No. 29, Seq. Id.No. 30, Seq. Id. No. 31, Seq. Id. No. 32, Seq.

Id. No. 33, Seq. Id. No. 34, Seq. Id. No. 35, Seq. Id. No. 36, Seq. Id.No. 37, Seq. Id. No. 38, Seq. Id. No. 39, Seq. Id. No. 40, Seq. Id. No.41, Seq. Id. No. 42, Seq. Id. No. 43, Seq. Id. No. 44, Seq. Id. No. 45,Seq. Id. No. 46, Seq. Id. No. 47, Seq. Id. No. 48, Seq. Id. No. 49, Seq.Id. No. 50, Seq. Id. No. 51, Seq. Id. No. 52, Seq. Id. No. 53, Seq. Id.No. 54, Seq. Id. No. 55, Seq. Id. No. 56, Seq. Id. No. 57, Seq. Id. No.58, Seq. Id. No. 59, Seq. Id. No. 60, Seq. Id. No. 61, Seq. Id. No. 62,Seq. Id. No. 63, Seq. Id. No. 65, Seq. Id. No. 66, Seq. Id. No. 67, Seq.Id. No. 68, Seq. Id. No. 69, Seq. Id. No. 70, Seq. Id. No. 71, Seq. Id.No. 72, Seq. Id. No. 73, Seq. Id. No. 74, Seq. Id. No. 75, Seq. Id. No.76, Seq. Id. No. 77, Seq. Id. No. 78, Seq. Id. No. 79, Seq. Id. No. 80,Seq. Id. No. 81, Seq. Id. No. 82, Seq. Id. No. 83, Seq. Id. No. 84, Seq.Id. No. 85, Seq. Id. No. 86, Seq. Id. No. 87, Seq. Id. No. 88, Seq. Id.No. 89, Seq. Id. No. 90, Seq. Id. No. 91, Seq. Id. No. 92, Seq. Id. No.93, Seq. Id. No. 94, Seq. Id. No. 95, Seq. Id. No. 96, Seq. Id. No. 97,Seq. Id. No. 98, Seq. Id. No. 99, Seq. Id. No. 100, Seq. Id. No. 101,Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No. 105, Seq. Id. No. 106,Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No. 109, Seq. Id. No. 110,Seq. Id. No. 111, Seq. Id. No. 112, Seq. Id. No. 113, Seq. Id. No. 114,Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No. 117, Seq. Id. No. 118,Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No. 121, Seq. Id. No. 122,Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No. 125, Seq. Id. No. 126,Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No. 129, Seq. Id. No. 130,Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No. 133, Seq. Id. No. 134,Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No. 137, Seq. Id. No. 138,Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No. 141, Seq. Id. No. 142,Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No. 145, Seq. Id. No. 146,Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No. 149, Seq. Id. No. 150,Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No. 153, Seq. Id. No. 154,Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No. 157, Seq. Id. No. 158,Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No. 161, Seq. Id. No. 162,Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No. 165, Seq. Id. No. 166,Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No. 169, Seq. Id. No. 170,Seq. Id. No. 171, Seq. Id. No. 172, Seq. Id. No. 173, Seq. Id. No. 174,Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No. 177, Seq. Id. No. 178,Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No. 181, Seq. Id. No. 182,Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No. 185, Seq. Id. No. 186,Seq. Id. No. 187, Seq. Id. No. 188, Seq. Id. No. 189, Seq. Id. No. 190,Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No. 193, Seq. Id. No. 194,Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No. 197, Seq. Id. No. 198,Seq. Id. No. 199, Seq. Id. No. 200, Seq. Id. No. 201, Seq. Id. No. 202,Seq. Id. No. 203, Seq. Id. No. 204, Seq. Id. No. 205, Seq. Id. No. 206,Seq. Id. No. 207, Seq. Id. No. 208, Seq. Id. No. 209, Seq. Id. No. 210,Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No. 213, Seq. Id. No. 214,Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No. 217, Seq. Id. No. 218,Seq. Id. No. 219, Seq. Id. No. 220, Seq. Id. No. 221, Seq. Id. No. 222,Seq. Id. No. 223, Seq. Id. No. 224, Seq. Id. No. 225, Seq. Id. No. 226,Seq. Id. No. 227, Seq. Id. No. 228, Seq. Id. No. 229, Seq. Id. No. 230,Seq. Id. No. 231, Seq. Id. No. 232, Seq. Id. No. 233, Seq. Id. No. 234,Seq. Id. No. 235, Seq. Id. No. 236, Seq. Id. No. 237, Seq. Id. No. 238,Seq. Id. No. 239, Seq. Id. No. 240, Seq. Id. No. 241, Seq. Id. No. 242,Seq. Id. No. 243, Seq. Id. No. 244, Seq. Id. No. 245, Seq. Id. No. 246,Seq. Id. No. 247, Seq. Id. No. 248, Seq. Id. No. 249, Seq. Id. No. 250,Seq. Id. No. 251, Seq. Id. No. 252, Seq. Id. No. 253, Seq. Id. No. 254,Seq. Id. No. 255, Seq. Id. No. 256, Seq. Id. No. 257, Seq. Id. No. 258,Seq. Id. No. 259, Seq. Id. No. 260, Seq. Id. No. 261, Seq. Id. No. 262,Seq. Id. No. 263, Seq. Id. No. 264, Seq. Id. No. 265, Seq. Id. No. 266,Seq. Id. No. 267, Seq. Id. No. 268, Seq. Id. No. 269, Seq. Id. No. 270,Seq. Id. No. 271, Seq. Id. No. 272, Seq. Id. No. 273, Seq. Id. No. 274,Seq. Id. No. 275, Seq. Id. No. 276, Seq. Id. No. 277, Seq. Id. No. 278,Seq. Id. No. 279, Seq. Id. No. 280, Seq. Id. No. 281, Seq. Id. No. 282,Seq. Id. No. 283, Seq. Id. No. 284, Seq. Id. No. 285, Seq. Id. No. 286,Seq. Id. No. 287, Seq. Id. No. 288, Seq. Id. No. 289, Seq. Id. No. 290,Seq. Id. No. 291, Seq. Id. No. 292, Seq. Id. No. 293, Seq. Id. No. 294,Seq. Id. No. 295, Seq. Id. No. 296, Seq. Id. No. 297, Seq. Id. No. 298,Seq. Id. No. 299, Seq. Id. No. 300, Seq. Id. No. 301, Seq. Id. No. 302,Seq. Id. No. 303, Seq. Id. No. 304, Seq. Id. No. 305, Seq. Id. No. 306,Seq. Id. No. 307, Seq. Id. No. 308, Seq. Id. No. 310, Seq. Id. No. 311,Seq. Id. No. 312, Seq. Id. No. 313, Seq. Id. No. 314, Seq. Id. No. 315,Seq. Id. No. 316, Seq. Id. No. 317, Seq. Id. No. 318, Seq. Id. No. 319,Seq. Id. No. 320, Seq. Id. No. 321, Seq. Id. No. 322, Seq. Id. No. 323,Seq. Id. No. 324, Seq. Id. No. 325, Seq. Id. No. 326, Seq. Id. No. 327,Seq. Id. No. 328, Seq. Id. No. 329, Seq. Id. No. 330, Seq. Id. No. 331,Seq. Id. No. 332, Seq. Id. No. 333, Seq. Id. No. 334, Seq. Id. No. 335,Seq. Id. No. 336, Seq. Id. No. 337, Seq. Id. No. 338, Seq. Id. No. 339,Seq. Id. No. 340, Seq. Id. No. 341, Seq. Id. No. 342, Seq. Id. No. 343,Seq. Id. No. 344, Seq. Id. No. 345, Seq. Id. No. 346, Seq. Id. No. 347,Seq. Id. No. 348, Seq. Id. No. 349, Seq. Id. No. 350, Seq. Id. No. 351,Seq. Id. No. 352, Seq. Id. No. 353, Seq. Id. No. 354, Seq. Id. No. 355,Seq. Id. No. 356, Seq. Id. No. 357, Seq. Id. No. 358, Seq. Id. No. 359,Seq. Id. No. 360, Seq. Id. No. 361, Seq. Id. No. 362, Seq. Id. No. 363,Seq. Id. No. 364, Seq. Id. No. 365, Seq. Id. No. 366, Seq. Id. No. 367,Seq. Id. No. 368, Seq. Id. No. 369, Seq. Id. No. 371, Seq. Id. No. 372,Seq. Id. No. 374, Seq. Id. No. 375, Seq. Id. No. 376, Seq. Id. No. 377,Seq. Id. No. 378, Seq. Id. No. 379, Seq. Id. No. 380, Seq. Id. No. 381,Seq. Id. No. 382, Seq. Id. No. 383, Seq. Id. No. 384, Seq. Id. No. 385,Seq. Id. No. 386, Seq. Id. No. 387, Seq. Id. No. 388, Seq. Id. No. 389,Seq. Id. No. 390, Seq. Id. No. 391, Seq. Id. No. 392, Seq. Id. No. 393,Seq. Id. No. 394, Seq. Id. No. 395, Seq. Id. No. 396, Seq. Id. No. 397,Seq. Id. No. 398, Seq. Id. No. 399, Seq. Id. No. 400, Seq. Id. No. 401,Seq. Id. No. 402, Seq. Id. No. 403, Seq. Id. No. 404, Seq. Id. No. 405,Seq. Id. No. 406, Seq. Id. No. 407, Seq. Id. No. 408, Seq. Id. No. 409,Seq. Id. No. 410, Seq. Id. No. 411, Seq. Id. No. 412, Seq. Id. No. 413,Seq. Id. No. 414, Seq. Id. No. 415, Seq. Id. No. 416, Seq. Id. No. 417,Seq. Id. No. 418, Seq. Id. No. 419, Seq. Id. No. 420, Seq. Id. No. 421,Seq. Id. No. 422, Seq. Id. No. 423, Seq. Id. No. 424, Seq. Id. No. 425,Seq. Id. No. 426, Seq. Id. No. 427, Seq. Id. No. 428, Seq. Id. No. 429,Seq. Id. No. 430, Seq. Id. No. 431, Seq. Id. No. 432, Seq. Id. No. 433,Seq. Id. No. 434, Seq. Id. No. 435, Seq. Id. No. 436, Seq. Id. No. 437,Seq. Id. No. 438, Seq. Id. No. 439, Seq. Id. No. 440, Seq. Id. No. 441,Seq. Id. No. 442, Seq. Id. No. 443, Seq. Id. No. 444, Seq. Id. No. 445,Seq. Id. No. 446, Seq. Id. No. 447, Seq. Id. No. 448, Seq. Id. No. 449,Seq. Id. No. 450, Seq. Id. No. 451, Seq. Id. No. 452, Seq. Id. No. 453,Seq. Id. No. 454, Seq. Id. No. 455, Seq. Id. No. 456, Seq. Id. No. 457,Seq. Id. No. 458, Seq. Id. No. 459, Seq. Id. No. 460, Seq. Id. No. 461,Seq. Id. No. 462, Seq. Id. No. 463, Seq. Id. No. 464, Seq. Id. No. 465,Seq. Id. No. 466, Seq. Id. No. 467, Seq. Id. No. 468, Seq. Id. No. 469,Seq. Id. No. 470, Seq. Id. No. 471, Seq. Id. No. 472, Seq. Id. No. 473,Seq. Id. No. 474, Seq. Id. No. 475, Seq. Id. No. 476, Seq. Id. No. 477,Seq. Id. No. 478, Seq. Id. No. 479, Seq. Id. No. 480, Seq. Id. No. 481,Seq. Id. No. 482, Seq. Id. No. 483, Seq. Id. No. 484, Seq. Id. No. 485,Seq. Id. No. 486, Seq. Id. No. 487, Seq. Id. No. 488, Seq. Id. No. 489,Seq. Id. No. 490, Seq. Id. No. 491, Seq. Id. No. 492, Seq. Id. No. 493,Seq. Id. No. 494, Seq. Id. No. 495, Seq. Id. No. 496, Seq. Id. No. 497,Seq. Id. No. 498, Seq. Id. No. 499, Seq. Id. No. 500, Seq. Id. No. 501,Seq. Id. No. 502, Seq. Id. No. 503, Seq. Id. No. 504, Seq. Id. No. 505,Seq. Id. No. 506, Seq. Id. No. 507, Seq. Id. No. 508, Seq. Id. No. 509,Seq. Id. No. 510, Seq. Id. No. 511, Seq. Id. No. 512, Seq. Id. No. 513,Seq. Id. No. 514, Seq. Id. No. 515, Seq. Id. No. 516 and Seq. Id. No.517.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the group consisting of Seq. Id. No. 518, Seq. Id. No.522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 526, Seq. Id. No.527, Seq. Id. No. 528, Seq. Id. No. 529, Seq. Id. No. 530, Seq. Id. No.531, Seq. Id. No. 532, Seq. Id. No. 533, Seq. Id. No. 534, Seq. Id. No.535, Seq. Id. No. 536, Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No.539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No.543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No.547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No.551, Seq. Id. No. 552, Seq. Id. No. 553, Seq. Id. No. 554, Seq. Id. No.555, Seq. Id. No. 557, Seq. Id. No. 559, Seq. Id. No. 560, Seq. Id. No.562, Seq. Id. No. 563, Seq. Id. No. 564, Seq. Id. No. 565, Seq. Id. No.567, Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No.571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No.575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No.579, Seq. Id. No. 580, Seq. Id. No. 581, Seq. Id. No. 582, Seq. Id. No.583, Seq. Id. No. 584, Seq. Id. No. 585, Seq. Id. No. 586, Seq. Id. No.587, Seq. Id. No. 588, Seq. Id. No. 589, Seq. Id. No. 590, Seq. Id. No.591, Seq. Id. No. 592, Seq. Id. No. 593, Seq. Id. No. 594, Seq. Id. No.595, Seq. Id. No. 596, Seq. Id. No. 597, Seq. Id. No. 598, Seq. Id. No.599 and Seq. Id. No. 601.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the group consisting of Seq. Id. No. 604, Seq. Id. No.605, Seq. Id. No. 606, Seq. Id. No. 607, Seq. Id. No. 608, Seq. Id. No.609, Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No.613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No.617, Seq. Id. No. 618 and Seq. Id. No. 619.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide that isselected from the group consisting of Seq. Id. No. 519, Seq. Id. No.520, Seq. Id. No. 521, and Seq. Id. No. 561.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the group consisting of Seq. Id. No. 554, Seq. Id. No.555, Seq. Id. No. 556, Seq. Id. No. 557, Seq. Id. No. 558, Seq. Id. No.559, and Seq. Id. No. 560.

A certain embodiment of present invention provides a method as describedherein, wherein the level of an autoantibody in the blood sample of thehuman subject is 5 times higher than the level of said autoantibodyrepresentative for a human subject of a healthy population.

A certain embodiment of present invention provides erlotinib or apharmaceutically acceptable salt thereof, in particular erlotinibhydrochloride, for use in treating a NSCLC patient identified by amethod as described herein comprising administering erlotinib or apharmaceutically acceptable salt thereof, in particular erlotinibhydrochloride to the patient.

A certain embodiment of present invention provides the use of anautoantibody for predicting the response of a NSCLC patient to erlotinibor a pharmaceutically acceptable salt thereof, in particular erlotinibhydrochloride, treatment, which antibody was identified by a method asdescribed herein.

A certain embodiment of present invention provides a kit for detectingin a blood sample of the human subject a level of one or moreautoantibodies selected from the group of autoantibodies recognizingmutated human EGFR, wherein an increased level of said autoantibodiesselected from the group of autoantibodies recognizing mutated human EGFRin the blood sample of the human subject compared to a level of saidautoantibodies representative for a human subject of a healthypopulation is indicative for non-small cell lung cancer.

A certain embodiment of present invention provides a kit as describedherein, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the group consisting of Seq. Id. No. 1, Seq. Id. No. 2,Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7 and Seq.Id. No. 15.

A certain embodiment of present invention provides a kit as describedherein, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the group consisting of Seq. Id. No. 16, Seq. Id. No.17, Seq. Id. No. 18, Seq. Id. No. 19, Seq. Id. No. 20, Seq. Id. No. 21,Seq. Id. No. 22, Seq. Id. No. 23, Seq. Id. No. 24, Seq. Id. No. 25, Seq.Id. No. 26, Seq. Id. No. 27, Seq. Id. No. 28, Seq. Id. No. 29, Seq. Id.No. 30, Seq. Id. No. 31, Seq. Id. No. 32, Seq. Id. No. 33, Seq. Id. No.34, Seq. Id. No. 35, Seq. Id. No. 36, Seq. Id. No. 37, Seq. Id. No. 38,Seq. Id. No. 39, Seq. Id. No. 40, Seq. Id. No. 41, Seq. Id. No. 42, Seq.Id. No. 43, Seq. Id. No. 44, Seq. Id. No. 45, Seq. Id. No. 46, Seq. Id.No. 47, Seq. Id. No. 48, Seq. Id. No. 49, Seq. Id. No. 50, Seq. Id. No.51, Seq. Id. No. 52, Seq. Id. No. 53, Seq. Id. No. 54, Seq. Id. No. 55,Seq. Id. No. 56, Seq. Id. No. 57, Seq. Id. No. 58, Seq. Id. No. 59, Seq.Id. No. 60, Seq. Id. No. 61, Seq. Id. No. 62, Seq. Id. No. 63, Seq. Id.No. 65, Seq. Id. No. 66, Seq. Id. No. 67, Seq. Id. No. 68, Seq. Id. No.69, Seq. Id. No. 70, Seq. Id. No. 71, Seq. Id. No. 72, Seq. Id. No. 73,Seq. Id. No. 74, Seq. Id. No. 75, Seq. Id. No. 76, Seq. Id. No. 77, Seq.Id. No. 78, Seq. Id. No. 79, Seq. Id. No. 80, Seq. Id. No. 81, Seq. Id.No. 82, Seq. Id. No. 83, Seq. Id. No. 84, Seq. Id. No. 85, Seq. Id. No.86, Seq. Id. No. 87, Seq. Id. No. 88, Seq. Id. No. 89, Seq. Id. No. 90,Seq. Id. No. 91, Seq. Id. No. 92, Seq. Id. No. 93, Seq. Id. No. 94, Seq.Id. No. 95, Seq. Id. No. 96, Seq. Id. No. 97, Seq. Id. No. 98, Seq. Id.No. 99, Seq. Id. No. 100, Seq. Id. No. 101, Seq. Id. No. 103, Seq. Id.No. 104, Seq. Id. No. 105, Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id.No. 108, Seq. Id. No. 109, Seq. Id. No. 110, Seq. Id. No. 111, Seq. Id.No. 112, Seq. Id. No. 113, Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id.No. 116, Seq. Id. No. 117, Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id.No. 120, Seq. Id. No. 121, Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id.No. 124, Seq. Id. No. 125, Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id.No. 128, Seq. Id. No. 129, Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id.No. 132, Seq. Id. No. 133, Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id.No. 136, Seq. Id. No. 137, Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id.No. 140, Seq. Id. No. 141, Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id.No. 144, Seq. Id. No. 145, Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id.No. 148, Seq. Id. No. 149, Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id.No. 152, Seq. Id. No. 153, Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id.No. 156, Seq. Id. No. 157, Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id.No. 160, Seq. Id. No. 161, Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id.No. 164, Seq. Id. No. 165, Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id.No. 168, Seq. Id. No. 169, Seq. Id. No. 170, Seq. Id. No. 171, Seq. Id.No. 172, Seq. Id. No. 173, Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id.No. 176, Seq. Id. No. 177, Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id.No. 180, Seq. Id. No. 181, Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id.No. 184, Seq. Id. No. 185, Seq. Id. No. 186, Seq. Id. No. 187, Seq. Id.No. 188, Seq. Id. No. 189, Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id.No. 192, Seq. Id. No. 193, Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id.No. 196, Seq. Id. No. 197, Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id.No. 200, Seq. Id. No. 201, Seq. Id. No. 202, Seq. Id. No. 203, Seq. Id.No. 204, Seq. Id. No. 205, Seq. Id. No. 206, Seq. Id. No. 207, Seq. Id.No. 208, Seq. Id. No. 209, Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id.No. 212, Seq. Id. No. 213, Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id.No. 216, Seq. Id. No. 217, Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id.No. 220, Seq. Id. No. 221, Seq. Id. No. 222, Seq. Id. No. 223, Seq. Id.No. 224, Seq. Id. No. 225, Seq. Id. No. 226, Seq. Id. No. 227, Seq. Id.No. 228, Seq. Id. No. 229, Seq. Id. No. 230, Seq. Id. No. 231, Seq. Id.No. 232, Seq. Id. No. 233, Seq. Id. No. 234, Seq. Id. No. 235, Seq. Id.No. 236, Seq. Id. No. 237, Seq. Id. No. 238, Seq. Id. No. 239, Seq. Id.No. 240, Seq. Id. No. 241, Seq. Id. No. 242, Seq. Id. No. 243, Seq. Id.No. 244, Seq. Id. No. 245, Seq. Id. No. 246, Seq. Id. No. 247, Seq. Id.No. 248, Seq. Id. No. 249, Seq. Id. No. 250, Seq. Id. No. 251, Seq. Id.No. 252, Seq. Id. No. 253, Seq. Id. No. 254, Seq. Id. No. 255, Seq. Id.No. 256, Seq. Id. No. 257, Seq. Id. No. 258, Seq. Id. No. 259, Seq. Id.No. 260, Seq. Id. No. 261, Seq. Id. No. 262, Seq. Id. No. 263, Seq. Id.No. 264, Seq. Id. No. 265, Seq. Id. No. 266, Seq. Id. No. 267, Seq. Id.No. 268, Seq. Id. No. 269, Seq. Id. No. 270, Seq. Id. No. 271, Seq. Id.No. 272, Seq. Id. No. 273, Seq. Id. No. 274, Seq. Id. No. 275, Seq. Id.No. 276, Seq. Id. No. 277, Seq. Id. No. 278, Seq. Id. No. 279, Seq. Id.No. 280, Seq. Id. No. 281, Seq. Id. No. 282, Seq. Id. No. 283, Seq. Id.No. 284, Seq. Id. No. 285, Seq. Id. No. 286, Seq. Id. No. 287, Seq. Id.No. 288, Seq. Id. No. 289, Seq. Id. No. 290, Seq. Id. No. 291, Seq. Id.No. 292, Seq. Id. No. 293, Seq. Id. No. 294, Seq. Id. No. 295, Seq. Id.No. 296, Seq. Id. No. 297, Seq. Id. No. 298, Seq. Id. No. 299, Seq. Id.No. 300, Seq. Id. No. 301, Seq. Id. No. 302, Seq. Id. No. 303, Seq. Id.No. 304, Seq. Id. No. 305, Seq. Id. No. 306, Seq. Id. No. 307, Seq. Id.No. 308, Seq. Id. No. 310, Seq. Id. No. 311, Seq. Id. No. 312, Seq. Id.No. 313, Seq. Id. No. 314, Seq. Id. No. 315, Seq. Id. No. 316, Seq. Id.No. 317, Seq. Id. No. 318, Seq. Id. No. 319, Seq. Id. No. 320, Seq. Id.No. 321, Seq. Id. No. 322, Seq. Id. No. 323, Seq. Id. No. 324, Seq. Id.No. 325, Seq. Id. No. 326, Seq. Id. No. 327, Seq. Id. No. 328, Seq. Id.No. 329, Seq. Id. No. 330, Seq. Id. No. 331, Seq. Id. No. 332, Seq. Id.No. 333, Seq. Id. No. 334, Seq. Id. No. 335, Seq. Id. No. 336, Seq. Id.No. 337, Seq. Id. No. 338, Seq. Id. No. 339, Seq. Id. No. 340, Seq. Id.No. 341, Seq. Id. No. 342, Seq. Id. No. 343, Seq. Id. No. 344, Seq. Id.No. 345, Seq. Id. No. 346, Seq. Id. No. 347, Seq. Id. No. 348, Seq. Id.No. 349, Seq. Id. No. 350, Seq. Id. No. 351, Seq. Id. No. 352, Seq. Id.No. 353, Seq. Id. No. 354, Seq. Id. No. 355, Seq. Id. No. 356, Seq. Id.No. 357, Seq. Id. No. 358, Seq. Id. No. 359, Seq. Id. No. 360, Seq. Id.No. 361, Seq. Id. No. 362, Seq. Id. No. 363, Seq. Id. No. 364, Seq. Id.No. 365, Seq. Id. No. 366, Seq. Id. No. 367, Seq. Id. No. 368, Seq. Id.No. 369, Seq. Id. No. 371, Seq. Id. No. 372, Seq. Id. No. 374, Seq. Id.No. 375, Seq. Id. No. 376, Seq. Id. No. 377, Seq. Id. No. 378, Seq. Id.No. 379, Seq. Id. No. 380, Seq. Id. No. 381, Seq. Id. No. 382, Seq. Id.No. 383, Seq. Id. No. 384, Seq. Id. No. 385, Seq. Id. No. 386, Seq. Id.No. 387, Seq. Id. No. 388, Seq. Id. No. 389, Seq. Id. No. 390, Seq. Id.No. 391, Seq. Id. No. 392, Seq. Id. No. 393, Seq. Id. No. 394, Seq. Id.No. 395, Seq. Id. No. 396, Seq. Id. No. 397, Seq. Id. No. 398, Seq. Id.No. 399, Seq. Id. No. 400, Seq. Id. No. 401, Seq. Id. No. 402, Seq. Id.No. 403, Seq. Id. No. 404, Seq. Id. No. 405, Seq. Id. No. 406, Seq. Id.No. 407, Seq. Id. No. 408, Seq. Id. No. 409, Seq. Id. No. 410, Seq. Id.No. 411, Seq. Id. No. 412, Seq. Id. No. 413, Seq. Id. No. 414, Seq. Id.No. 415, Seq. Id. No. 416, Seq. Id. No. 417, Seq. Id. No. 418, Seq. Id.No. 419, Seq. Id. No. 420, Seq. Id. No. 421, Seq. Id. No. 422, Seq. Id.No. 423, Seq. Id. No. 424, Seq. Id. No. 425, Seq. Id. No. 426, Seq. Id.No. 427, Seq. Id. No. 428, Seq. Id. No. 429, Seq. Id. No. 430, Seq. Id.No. 431, Seq. Id. No. 432, Seq. Id. No. 433, Seq. Id. No. 434, Seq. Id.No. 435, Seq. Id. No. 436, Seq. Id. No. 437, Seq. Id. No. 438, Seq. Id.No. 439, Seq. Id. No. 440, Seq. Id. No. 441, Seq. Id. No. 442, Seq. Id.No. 443, Seq. Id. No. 444, Seq. Id. No. 445, Seq. Id. No. 446, Seq. Id.No. 447, Seq. Id. No. 448, Seq. Id. No. 449, Seq. Id. No. 450, Seq. Id.No. 451, Seq. Id. No. 452, Seq. Id. No. 453, Seq. Id. No. 454, Seq. Id.No. 455, Seq. Id. No. 456, Seq. Id. No. 457, Seq. Id. No. 458, Seq. Id.No. 459, Seq. Id. No. 460, Seq. Id. No. 461, Seq. Id. No. 462, Seq. Id.No. 463, Seq. Id. No. 464, Seq. Id. No. 465, Seq. Id. No. 466, Seq. Id.No. 467, Seq. Id. No. 468, Seq. Id. No. 469, Seq. Id. No. 470, Seq. Id.No. 471, Seq. Id. No. 472, Seq. Id. No. 473, Seq. Id. No. 474, Seq. Id.No. 475, Seq. Id. No. 476, Seq. Id. No. 477, Seq. Id. No. 478, Seq. Id.No. 479, Seq. Id. No. 480, Seq. Id. No. 481, Seq. Id. No. 482, Seq. Id.No. 483, Seq. Id. No. 484, Seq. Id. No. 485, Seq. Id. No. 486, Seq. Id.No. 487, Seq. Id. No. 488, Seq. Id. No. 489, Seq. Id. No. 490, Seq. Id.No. 491, Seq. Id. No. 492, Seq. Id. No. 493, Seq. Id. No. 494, Seq. Id.No. 495, Seq. Id. No. 496, Seq. Id. No. 497, Seq. Id. No. 498, Seq. Id.No. 499, Seq. Id. No. 500, Seq. Id. No. 501, Seq. Id. No. 502, Seq. Id.No. 503, Seq. Id. No. 504, Seq. Id. No. 505, Seq. Id. No. 506, Seq. Id.No. 507, Seq. Id. No. 508, Seq. Id. No. 509, Seq. Id. No. 510, Seq. Id.No. 511, Seq. Id. No. 512, Seq. Id. No. 513, Seq. Id. No. 514, Seq. Id.No. 515, Seq. Id. No. 516 and Seq. Id. No. 517.

A certain embodiment of present invention provides a kit as describedherein, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the group consisting of Seq. Id. No. 518, Seq. Id. No.522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 526, Seq. Id. No.527, Seq. Id. No. 528, Seq. Id. No. 529, Seq. Id. No. 530, Seq. Id. No.531, Seq. Id. No. 532, Seq. Id. No. 533, Seq. Id. No. 534, Seq. Id. No.535, Seq. Id. No. 536, Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No.539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No.543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No.547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No.551, Seq. Id. No. 552, Seq. Id. No. 553, Seq. Id. No. 554, Seq. Id. No.555, Seq. Id. No. 557, Seq. Id. No. 559, Seq. Id. No. 560, Seq. Id. No.562, Seq. Id. No. 563, Seq. Id. No. 564, Seq. Id. No. 565, Seq. Id. No.567, Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No.571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No.575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No.579, Seq. Id. No. 580, Seq. Id. No. 581, Seq. Id. No. 582, Seq. Id. No.583, Seq. Id. No. 584, Seq. Id. No. 585, Seq. Id. No. 586, Seq. Id. No.587, Seq. Id. No. 588, Seq. Id. No. 589, Seq. Id. No. 590, Seq. Id. No.591, Seq. Id. No. 592, Seq. Id. No. 593, Seq. Id. No. 594, Seq. Id. No.595, Seq. Id. No. 596, Seq. Id. No. 597, Seq. Id. No. 598, Seq. Id. No.599 and Seq. Id. No. 601.

A certain embodiment of present invention provides a kit as describedherein, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the group consisting of Seq. Id. No. 604, Seq. Id. No.605, Seq. Id. No. 606, Seq. Id. No. 607, Seq. Id. No. 608, Seq. Id. No.609, Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No.613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No.617, Seq. Id. No. 618 and Seq. Id. No. 619.

A certain embodiment of present invention provides a kit as describedherein, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the group consisting of Seq. Id. No. 554, Seq. Id. No.555, Seq. Id. No. 556, Seq. Id. No. 557, Seq. Id. No. 558, Seq. Id. No.559, and Seq. Id. No. 560.

A certain embodiment of present invention provides a kit as describedherein, wherein the autoantibody recognizes an EGFR peptide that isselected from the group consisting of Seq. Id. No. 519, Seq. Id. No.520, Seq. Id. No. 521, and Seq. Id. No. 561.

A certain embodiment of present invention provides a method of diagnosisof non-small cell lung cancer in a human subject comprising:

measuring in a blood sample of the human subject a level of anautoantibody selected from the group of autoantibodies recognizing humanEGFR, wherein an increased level of said autoantibody selected from thegroup of autoantibodies recognizing human EGFR in the blood sample ofthe human subject compared to a level of said autoantibodyrepresentative for a human subject of a healthy population is indicativefor non-small cell lung cancer, in particular wherein the level of anautoantibody recognizing human EGFR is measured.

Present invention provides a method of diagnosis of non-small cell lungcancer in a human subject comprising:

a) measuring in a blood sample of the human subject a level of anautoantibody selected from the group of autoantibodies recognizing humanEGFR,

b) comparing the level of said autoantibody to a reference level, and

c) providing a diagnosis of non-small cell lung cancer when the level ofsaid autoantibody is above the reference level.

Present invention provides a method of diagnosis of non-small cell lungcancer in a human subject comprising:

a) measuring in a blood sample of the human subject a level of anautoantibody selected from the group of autoantibodies recognizingmutated human EGFR,

b) comparing the level of said autoantibody to a reference level, and

c) recommending a treatment when the level of said autoantibody is abovethe reference level.

A certain embodiment of the present invention provides a method asdescribed above, wherein said treatment is erlotinib.

A certain embodiment of present invention provides a method as describedabove, wherein the autoantibody recognizes an EGFR peptide is selectedfrom the group consisting of Seq. Id. No. 1, Seq. Id. No. 2, Seq. Id.No. 3, Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7,Seq. Id. No. 8, Seq. Id. No. 9, Seq. Id. No. 10, Seq. Id. No. 11, Seq.Id. No. 12, Seq. Id. No. 13, Seq. Id. No. 14, Seq. Id. No. 15, Seq. Id.No. 16, Seq. Id. No. 17, Seq. Id. No. 18, Seq. Id. No. 19, Seq. Id. No.20, Seq. Id. No. 21, Seq. Id. No. 22, Seq. Id. No. 23, Seq. Id. No. 24,Seq. Id. No. 25, Seq. Id. No. 26, Seq. Id. No. 27, Seq. Id. No. 28, Seq.Id. No. 29, Seq. Id. No. 30, Seq. Id. No. 31, Seq. Id. No. 32, Seq. Id.No. 33, Seq. Id. No. 34, Seq. Id. No. 35, Seq. Id. No. 36, Seq. Id. No.37, Seq. Id. No. 38, Seq. Id. No. 39, Seq. Id. No. 40, Seq. Id. No. 41,Seq. Id. No. 42, Seq. Id. No. 43, Seq. Id. No. 44, Seq. Id. No. 45, Seq.Id. No. 46, Seq. Id. No. 47, Seq. Id. No. 48, Seq. Id. No. 49, Seq. Id.No. 50, Seq. Id. No. 51, Seq. Id. No. 52, Seq. Id. No. 53, Seq. Id. No.54, Seq. Id. No. 55, Seq. Id. No. 56, Seq. Id. No. 57, Seq. Id. No. 58,Seq. Id. No. 59, Seq. Id. No. 60, Seq. Id. No. 61, Seq. Id. No. 62, Seq.Id. No. 63, Seq. Id. No. 64, Seq. Id. No. 65, Seq. Id. No. 66, Seq. Id.No. 67, Seq. Id. No. 68, Seq. Id. No. 69, Seq. Id. No. 70, Seq. Id. No.71, Seq. Id. No. 72, Seq. Id. No. 73, Seq. Id. No. 74, Seq. Id. No. 75,Seq. Id. No. 76, Seq.

Id. No. 77, Seq. Id. No. 78, Seq. Id. No. 79, Seq. Id. No. 80, Seq. Id.No. 81, Seq. Id. No. 82, Seq. Id. No. 83, Seq. Id. No. 84, Seq. Id. No.85, Seq. Id. No. 86, Seq. Id. No. 87, Seq. Id. No. 88, Seq. Id. No. 89,Seq. Id. No. 90, Seq. Id. No. 91, Seq. Id. No. 92, Seq. Id. No. 93, Seq.Id. No. 94, Seq. Id. No. 95, Seq. Id. No. 96, Seq. Id. No. 97, Seq. Id.No. 98, Seq. Id. No. 99, Seq. Id. No. 100, Seq. Id. No. 101, Seq. Id.No. 102, Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No. 105, Seq. Id.No. 106, Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No. 109, Seq. Id.No. 110, Seq. Id. No. 111, Seq. Id. No. 112, Seq. Id. No. 113, Seq. Id.No. 114, Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No. 117, Seq. Id.No. 118, Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No. 121, Seq. Id.No. 122, Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No. 125, Seq. Id.No. 126, Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No. 129, Seq. Id.No. 130, Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No. 133, Seq. Id.No. 134, Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No. 137, Seq. Id.No. 138, Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No. 141, Seq. Id.No. 142, Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No. 145, Seq. Id.No. 146, Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No. 149, Seq. Id.No. 150, Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No. 153, Seq. Id.No. 154, Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No. 157, Seq. Id.No. 158, Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No. 161, Seq. Id.No. 162, Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No. 165, Seq. Id.No. 166, Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No. 169, Seq. Id.No. 170, Seq. Id. No. 171, Seq. Id. No. 172, Seq. Id. No. 173, Seq. Id.No. 174, Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No. 177, Seq. Id.No. 178, Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No. 181, Seq. Id.No. 182, Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No. 185, Seq. Id.No. 186, Seq. Id. No. 187, Seq. Id. No. 188, Seq. Id. No. 189, Seq. Id.No. 190, Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No. 193, Seq. Id.No. 194, Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No. 197, Seq. Id.No. 198, Seq. Id. No. 199, Seq. Id. No. 200, Seq. Id. No. 201, Seq. Id.No. 202, Seq. Id. No. 203, Seq. Id. No. 204, Seq. Id. No. 205, Seq. Id.No. 206, Seq. Id. No. 207, Seq. Id. No. 208, Seq. Id. No. 209, Seq. Id.No. 210, Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No. 213, Seq. Id.No. 214, Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No. 217, Seq. Id.No. 218, Seq. Id. No. 219, Seq. Id. No. 220, Seq. Id. No. 221, Seq. Id.No. 222, Seq. Id. No. 223, Seq. Id. No. 224, Seq. Id. No. 225, Seq. Id.No. 226, Seq. Id. No. 227, Seq. Id. No. 228, Seq. Id. No. 229, Seq. Id.No. 230, Seq. Id. No. 231, Seq. Id. No. 232, Seq. Id. No. 233, Seq. Id.No. 234, Seq. Id. No. 235, Seq. Id. No. 236, Seq. Id. No. 237, Seq. Id.No. 238, Seq. Id. No. 239, Seq. Id. No. 240, Seq. Id. No. 241, Seq. Id.No. 242, Seq. Id. No. 243, Seq. Id. No. 244, Seq. Id. No. 245, Seq. Id.No. 246, Seq. Id. No. 247, Seq. Id. No. 248, Seq. Id. No. 249, Seq. Id.No. 250, Seq. Id. No. 251, Seq. Id. No. 252, Seq. Id. No. 253, Seq. Id.No. 254, Seq. Id. No. 255, Seq. Id. No. 256, Seq. Id. No. 257, Seq. Id.No. 258, Seq. Id. No. 259, Seq. Id. No. 260, Seq. Id. No. 261, Seq. Id.No. 262, Seq. Id. No. 263, Seq. Id. No. 264, Seq. Id. No. 265, Seq. Id.No. 266, Seq. Id. No. 267, Seq. Id. No. 268, Seq. Id. No. 269, Seq. Id.No. 270, Seq. Id. No. 271, Seq. Id. No. 272, Seq. Id. No. 273, Seq. Id.No. 274, Seq. Id. No. 275, Seq. Id. No. 276, Seq. Id. No. 277, Seq. Id.No. 278, Seq. Id. No. 279, Seq. Id. No. 280, Seq. Id. No. 281, Seq. Id.No. 282, Seq. Id. No. 283, Seq. Id. No. 284, Seq. Id. No. 285, Seq. Id.No. 286, Seq. Id. No. 287, Seq. Id. No. 288, Seq. Id. No. 289, Seq. Id.No. 290, Seq. Id. No. 291, Seq. Id. No. 292, Seq. Id. No. 293, Seq. Id.No. 294, Seq. Id. No. 295, Seq. Id. No. 296, Seq. Id. No. 297, Seq. Id.No. 298, Seq. Id. No. 299, Seq. Id. No. 300, Seq. Id. No. 301, Seq. 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Id.No. 414, Seq. Id. No. 415, Seq. Id. No. 416, Seq. Id. No. 417, Seq. Id.No. 418, Seq. Id. No. 419, Seq. Id. No. 420, Seq. Id. No. 421, Seq. Id.No. 422, Seq. Id. No. 423, Seq. Id. No. 424, Seq. Id. No. 425, Seq. Id.No. 426, Seq. Id. No. 427, Seq. Id. No. 428, Seq. Id. No. 429, Seq. Id.No. 430, Seq. Id. No. 431, Seq. Id. No. 432, Seq. Id. No. 433, Seq. Id.No. 434, Seq. Id. No. 435, Seq. Id. No. 436, Seq. Id. No. 437, Seq. Id.No. 438, Seq. Id. No. 439, Seq. Id. No. 440, Seq. Id. No. 441, Seq. Id.No. 442, Seq. Id. No. 443, Seq. Id. No. 444, Seq. Id. No. 445, Seq. Id.No. 446, Seq. Id. No. 447, Seq. Id. No. 448, Seq. Id. No. 449, Seq. Id.No. 450, Seq. Id. No. 451, Seq. Id. No. 452, Seq. Id. No. 453, Seq. Id.No. 454, Seq. Id. No. 455, Seq. Id. No. 456, Seq. Id. No. 457, Seq. Id.No. 458, Seq. Id. No. 459, Seq. Id. No. 460, Seq. Id. No. 461, Seq. Id.No. 462, Seq. Id. No. 463, Seq. Id. No. 464, Seq. Id. No. 465, Seq. Id.No. 466, Seq. Id. No. 467, Seq. Id. No. 468, Seq. Id. No. 469, Seq. Id.No. 470, Seq. Id. No. 471, Seq. Id. No. 472, Seq. Id. No. 473, Seq. Id.No. 474, Seq. Id. No. 475, Seq. Id. No. 476, Seq. Id. No. 477, Seq. Id.No. 478, Seq. Id. No. 479, Seq. Id. No. 480, Seq. Id. No. 481, Seq. Id.No. 482, Seq. Id. No. 483, Seq. Id. No. 484, Seq. Id. No. 485, Seq. Id.No. 486, Seq. Id. No. 487, Seq. Id. No. 488, Seq. Id. No. 489, Seq. Id.No. 490, Seq. Id. No. 491, Seq. Id. No. 492, Seq. Id. No. 493, Seq. Id.No. 494, Seq. Id. No. 495, Seq. Id. No. 496, Seq. Id. No. 497, Seq. Id.No. 498, Seq. Id. No. 499, Seq. Id. No. 500, Seq. Id. No. 501, Seq. Id.No. 502, Seq. Id. No. 503, Seq. Id. No. 504, Seq. Id. No. 505, Seq. Id.No. 506, Seq. Id. No. 507, Seq. Id. No. 508, Seq. Id. No. 509, Seq. Id.No. 510, Seq. Id. No. 511, Seq. Id. No. 512, Seq. Id. No. 513, Seq. Id.No. 514, Seq. Id. No. 515, Seq. Id. No. 516, Seq. Id. No. 517, Seq. Id.No. 518, Seq. Id. No. 519, Seq. Id. No. 520, Seq. Id. No. 521, Seq. Id.No. 522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 525, Seq. Id.No. 526, Seq. Id. No. 527, Seq. Id. No. 528, Seq. Id. No. 529, Seq. Id.No. 530, Seq. Id. No. 531, Seq. Id. No. 532, Seq. Id. No. 533, Seq. Id.No. 534, Seq. Id. No. 535, Seq. Id. No. 536, Seq. Id. No. 537, Seq. Id.No. 538, Seq. Id. No. 539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id.No. 542, Seq. Id. No. 543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id.No. 546, Seq. Id. No. 547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id.No. 550, Seq. Id. No. 551, Seq. Id. No. 552, Seq. Id. No. 553, Seq. Id.No. 554, Seq. Id. No. 555, Seq. Id. No. 556, Seq. Id. No. 557, Seq. Id.No. 558, Seq. Id. No. 559, Seq. Id. No. 560, Seq. Id. No. 561, Seq. Id.No. 562, Seq. Id. No. 563, Seq. Id. No. 564, Seq. Id. No. 565, Seq. Id.No. 566, Seq. Id. No. 567, Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id.No. 570, Seq. Id. No. 571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id.No. 574, Seq. Id. No. 575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id.No. 578, Seq. Id. No. 579, Seq. Id. No. 580, Seq. Id. No. 581, Seq. Id.No. 582, Seq. Id. No. 583, Seq. Id. No. 584, Seq. Id. No. 585, Seq. Id.No. 586, Seq. Id. No. 587, Seq. Id. No. 588, Seq. Id. No. 589, Seq. Id.No. 590, Seq. Id. No. 591, Seq. Id. No. 592, Seq. Id. No. 593, Seq. Id.No. 594, Seq. Id. No. 595, Seq. Id. No. 596, Seq. Id. No. 597, Seq. Id.No. 598, Seq. Id. No. 599, Seq. Id. No. 600, Seq. Id. No. 601, Seq. Id.No. 602, Seq. Id. No. 603, Seq. Id. No. 604, Seq. Id. No. 605, Seq. Id.No. 606, Seq. Id. No. 607, Seq. Id. No. 608, Seq. Id. No. 609, Seq. Id.No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No. 613, Seq. Id.No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No. 617, Seq. Id.No. 618, and Seq. Id. No. 619.

A certain embodiment of present invention provides a method as describedabove, wherein the autoantibody recognizes an EGFR peptide that isselected from the group consisting of Seq. Id. No. 3, Seq. Id. No. 8,Seq. Id. No. 9, Seq. Id. No. 10, Seq. Id. No. 11, Seq. Id. No. 12, Seq.Id. No. 13, Seq. Id. No. 14, Seq. Id. No. 64, Seq. Id. No. 102, Seq. Id.No. 309, Seq. Id. No. 370, Seq. Id. No. 373, Seq. Id. No. 519, Seq. Id.No. 520, Seq. Id. No. 521, Seq. Id. No. 525, Seq. Id. No. 556, Seq. Id.No. 558, Seq. Id. No. 561, Seq. Id. No. 566, Seq. Id. No. 600, Seq. Id.No. 602 and Seq. Id. No. 603.

A certain embodiment of present invention provides a kit for detectingin a blood sample of the human subject a level of one or moreautoantibodies selected from the group of autoantibodies recognizinghuman EGFR, wherein an increased level of said autoantibodies selectedfrom the group of autoantibodies recognizing human EGFR in the bloodsample of the human subject compared to a level of said autoantibodiesrepresentative for a human subject of a healthy population is indicativefor non-small cell lung cancer.

A certain embodiment of present invention provides a kit as describedabove, wherein the autoantibody recognizes an EGFR peptide that isselected from the group consisting of Seq. Id. No. 3, Seq. Id. No. 8,Seq. Id. No. 9, Seq. Id. No. 10, Seq. Id. No. 11, Seq. Id. No. 12, Seq.Id. No. 13, Seq. Id. No. 14, Seq. Id. No. 64, Seq. Id. No. 102, Seq. Id.No. 309, Seq. Id. No. 370, Seq. Id. No. 373, Seq. Id. No. 519, Seq. Id.No. 520, Seq. Id. No. 521, Seq. Id. No. 525, Seq. Id. No. 556, Seq. Id.No. 558, Seq. Id. No. 561, Seq. Id. No. 566, Seq. Id. No. 600, Seq. Id.No. 602 and Seq. Id. No. 603.

A certain embodiment of present invention provides a kit according asdescribed herein, wherein the autoantibody recognizes a mutated EGFRpeptide that is selected from Seq. Id. No. 554, Seq. Id. No. 555, Seq.Id. No. 556, Seq. Id. No. 557, Seq. Id. No. 558, Seq. Id. No. 559, andSeq. Id. No. 560.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 518.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 519.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 520.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 521.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 522.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 523.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 524.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 525.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 526.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 527.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 528.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 529.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 530.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 531.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 532.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 533.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 534.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 535.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 536.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 537.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 538.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 539.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 540.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 541.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 542.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 543.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 544.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 545.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 546.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 547.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 548.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 549.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 550.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 551.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 552.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 553.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 554.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 555.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 556.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 557.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 558.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 559.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 560.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 561.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 562.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 563.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 564.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 565.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 566.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 567.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 568.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 569.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 570.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 571.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 572.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 573.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 574.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 575.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 576.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 577.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 578.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 579.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 580.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 581.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 582.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 583.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 584.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 585.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 586.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 587.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 588.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 589.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 590.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 591.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 592.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 593.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 594.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 595.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 596.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 597.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 598.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 599.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 600.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 601.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 602.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 603.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 604.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 605.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 606.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 607.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 608.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 609.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 610.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 611.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 612.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 613.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 614.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 615.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 616.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 617.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 618.

A certain embodiment of present invention provides a method as describedherein, wherein the autoantibody recognizes an EGFR peptide of Seq. Id.No. 619.

A certain embodiment of present invention provides a method as describedherein, wherein the level of an autoantibody recognizing p53 ismeasured.

A certain embodiment of present invention provides a method as describedherein, wherein the level of an autoantibody in the blood sample of thehuman subject is 5 times higher than the level of said autoantibodyrepresentative for a human subject of a healthy population.

A certain embodiment of present invention provides a method fordetermining the EGFR mutation status in a tumor tissue of a humansubject suffering from non-small cell lung cancer comprising:

detecting in a blood sample of a human being suffering from non-smallcell lung cancer an autoantibody selected from the group ofautoantibodies recognizing an human EGFR peptide as described herein,wherein the presence of said autoantibody is indicative for the presenceof a mutation of exon 19 in the gene encoding EGFR in human tissue.

A certain embodiment of present invention provides a method fordetermining the EGFR mutation status in a tumor tissue of a humansubject suffering from non-small cell lung cancer comprising:

detecting in a blood sample of a human being suffering from non-smallcell lung cancer an autoantibody selected from the group ofautoantibodies recognizing an human EGFR peptide as described herein,wherein the presence of said autoantibody is indicative for the presenceof a deletion of exon 21 in the gene encoding EGFR in human tissue.

A certain embodiment of present invention provides erlotinib or apharmaceutically acceptable salt thereof, in particular erlotinibhydrochloride, for use in treating a NSCLC patient identified by amethod as described herein comprising administering erlotinib or apharmaceutically acceptable salt thereof, in particular erlotinibhydrochloride to the patient.

A certain embodiment of present invention provides the use of anautoantibody for predicting the response of a NSCLC patient to erlotinibor a pharmaceutically acceptable salt thereof, in particular erlotinibhydrochloride, treatment, which antibody was identified by a method asdescribed herein.

A certain embodiment of present invention provides a kit for detectingin a blood sample of the human subject a level of one or moreautoantibodies selected from the group of autoantibodies recognizinghuman EGFR, wherein an increased level of said autoantibodies selectedfrom the group of autoantibodies recognizing human EGFR in the bloodsample of the human subject compared to a level of said autoantibodiesrepresentative for a human subject of a healthy population is indicativefor non-small cell lung cancer.

A certain embodiment of present invention provides a kit according asdescribed herein, wherein the autoantibody recognizes an EGFR peptidethat is selected from Seq. Id. No. 1-Seq. Id. No. 15.

A certain embodiment of present invention provides a kit according asdescribed herein, wherein the autoantibody recognizes an EGFR peptidethat is selected from Seq. Id. No. 16-Seq. Id. No. 517.

A certain embodiment of present invention provides a kit according asdescribed herein, wherein the autoantibody recognizes an EGFR peptidethat is selected from Seq. Id. No. 518-Seq. Id. No. 602.

A certain embodiment of present invention provides a kit according asdescribed herein, wherein the autoantibody recognizes an EGFR peptidethat is selected from Seq. Id. No. 603-Seq. Id. No. 619.

A certain embodiment of present invention provides a kit according asdescribed herein, wherein the autoantibody recognizes an EGFR peptidethat is selected from Seq. Id. No. 519, Seq. Id. No. 520, Seq. Id. No.521, and Seq. Id. No. 561.

FIGURES AND SEQ. ID. NUMBERS

FIG. 1: Log-transformed values of peptide binding for all peptidesgrouped by patient (49 samples, 4-digit numbers) and controls (1-digitnumbers). Patients have on average higher signals, with a number ofantibodies binding to peptides stronger than any signal in control sera.

FIG. 2: Histogram of distribution of the p-values for the significanceof the antibody titers in a Cox regression model of OS orPFS˜EGFR+TRT+EGFR:TRT+SEX+Antibody titer+ Antibody titer:TRT. Ifantibody titers do not affect survival, a uniform distribution isexpected. The deviation from the uniform distribution is highlysignificant, which lead to the conclusion that approximately 50% of the245 antibody titers with p-values<0.05 will have a significant influenceon progression free survival. A similar model calculation for overallsurvival (OS) yields 663 candidates at p-Values<0.05 with a slightlybetter false discovery rate of approximately 40%.

FIG. 3: Venn diagrams illustrating the refinement of peptide candidatesfor (A) progression free survival and (B) overall survival. Pep—initialpeptide selection form Cox-regression model with all covariates; RASH:overlapping sequences from peptides predicting PFS (OS) in patients withrash in a proportional hazard model; TRT: overlapping sequences frompeptides predicting PFS (OS) in the erlotinib subgroup; EC4: univariatetest of response (after 4 cycles, categorical) vs. antibody titer.

FIG. 4: Presence of autoantibodies against a mutated EGFR peptidepredicts (p=0.006) a better treatment outcome in the Tarceva® arm of thetrial independent of the occurrence of rash.

TABLE 4  Seq. Id. No. 1-619 of human EGFR peptides, *indicates mutated EGFR Seq. Id. No. protein sequence   1*GEKVKIPVAIKPKANK   2* GEKVKIPVAIKSPKANK  3 KEYHAEGGKVPIKWM   4*KIPVAIKHPTSPK   5* KIPVAIKKPTSPK   6* KIPVAIKPTSPK   7* KIPVAIKRPTSPK  8MPFGCLLDYVREH  9 NSTFDSPAHWAQKGSHQI 10 PREFVENSECIQCHPECL 11RGPDNCIQCAHYIDGPHCVKTCP 12 RMHLPSPTDSNFYRA 13 SIDDTFLPVPEYIN 14VRKCKKCEGPCRKV  15* VRKSKKSEGPSRKV  16* ADKDILDEAYVMACA  17*ADKDILDEAYVMACG  18* ADKDILDEAYVMACV  19* ADKDILDEAYVMAIA  20*ADKDILDEAYVMAIG  21* ADKDILDEAYVMAIV  22* ADKDILDEAYVMANA  23*ADKDILDEAYVMANG  24* ADKDILDEAYVMANV  25* ADKDILDEAYVMASA  26*ADKDILDEAYVMASG  27* ADKDILDEAYVMASV  28* ADKDILDEAYVMTCA  29*ADKDILDEAYVMTCG  30* ADKDILDEAYVMTCV  31* ADKDILDEAYVMTIA  32*ADKDILDEAYVMTIG  33* ADKDILDEAYVMTIV  34* ADKDILDEAYVMTNA  35*ADKDILDEAYVMTNG  36* ADKDILDEAYVMTNV  37* ADKDILDEAYVMTSA  38*ADKDILDEAYVMTSG  39* ADKDILDEAYVMTSV  40* ADKEILDEAYVMACA  41*ADKEILDEAYVMACG  42* ADKEILDEAYVMACV  43* ADKEILDEAYVMAIA  44*ADKEILDEAYVMAIG  45* ADKEILDEAYVMAIV  46* ADKEILDEAYVMANA  47*ADKEILDEAYVMANG  48* ADKEILDEAYVMANV  49* ADKEILDEAYVMASA  50*ADKEILDEAYVMASG  51* ADKEILDEAYVMASV  52* ADKEILDEAYVMTCA  53*ADKEILDEAYVMTCG  54* ADKEILDEAYVMTCV  55* ADKEILDEAYVMTIA  56*ADKEILDEAYVMTIG  57* ADKEILDEAYVMTIV  58* ADKEILDEAYVMTNA  59*ADKEILDEAYVMTNG  60* ADKEILDEAYVMTNV  61* ADKEILDEAYVMTSA  62*ADKEILDEAYVMTSG  63* ADKEILDEAYVMTSV 64 AIKELREATSPKA  65* AIKILREATSPKA 66* AIKVLREATSPKA  67* ANKDILDEAYVMACA  68* ANKDILDEAYVMACG  69*ANKDILDEAYVMACV  70* ANKDILDEAYVMAIA  71* ANKDILDEAYVMAIG  72*ANKDILDEAYVMAIV  73* ANKDILDEAYVMANA  74* ANKDILDEAYVMANG  75*ANKDILDEAYVMANV  76* ANKDILDEAYVMASA  77* ANKDILDEAYVMASG  78*ANKDILDEAYVMASV  79* ANKDILDEAYVMTCA  80* ANKDILDEAYVMTCG  81*ANKDILDEAYVMTCV  82* ANKDILDEAYVMTIA  83* ANKDILDEAYVMTIG  84*ANKDILDEAYVMTIV  85* ANKDILDEAYVMTNA  86* ANKDILDEAYVMTNG  87*ANKDILDEAYVMTNV  88* ANKDILDEAYVMTSA  89* ANKDILDEAYVMTSG  90*ANKDILDEAYVMTSV  91* ANKEILDEAYVMACA  92* ANKEILDEAYVMACG  93*ANKEILDEAYVMACV  94* ANKEILDEAYVMAIA  95* ANKEILDEAYVMAIG  96*ANKEILDEAYVMAIV  97* ANKEILDEAYVMANA  98* ANKEILDEAYVMANG  99*ANKEILDEAYVMANV 100* ANKEILDEAYVMASA 101* ANKEILDEAYVMASG 102 ANKEILDEAYVMASV 103* ANKEILDEAYVMTCA 104* ANKEILDEAYVMTCG 105*ANKEILDEAYVMTCV 106* ANKEILDEAYVMTIA 107* ANKEILDEAYVMTIG 108*ANKEILDEAYVMTIV 109* ANKEILDEAYVMTNA 110* ANKEILDEAYVMTNG 111*ANKEILDEAYVMTNV 112* ANKEILDEAYVMTSA 113* ANKEILDEAYVMTSG 114*ANKEILDEAYVMTSV 115* ASKDILDEAYVMACA 116* ASKDILDEAYVMACG 117*ASKDILDEAYVMACV 118* ASKDILDEAYVMAIA 119* ASKDILDEAYVMAIG 120*ASKDILDEAYVMAIV 121* ASKDILDEAYVMANA 122* ASKDILDEAYVMANG 123*ASKDILDEAYVMANV 124* ASKDILDEAYVMASA 125* ASKDILDEAYVMASG 126*ASKDILDEAYVMASV 127* ASKDILDEAYVMTCA 128* ASKDILDEAYVMTCG 129*ASKDILDEAYVMTCV 130* ASKDILDEAYVMTIA 131* ASKDILDEAYVMTIG 132*ASKDILDEAYVMTIV 133* ASKDILDEAYVMTNA 134* ASKDILDEAYVMTNG 135*ASKDILDEAYVMTNV 136* ASKDILDEAYVMTSA 137* ASKDILDEAYVMTSG 138*ASKDILDEAYVMTSV 139* ASKEILDEAYVMACA 140* ASKEILDEAYVMACG 141*ASKEILDEAYVMACV 142* ASKEILDEAYVMAIA 143* ASKEILDEAYVMAIG 144*ASKEILDEAYVMAIV 145* ASKEILDEAYVMANA 146* ASKEILDEAYVMANG 147*ASKEILDEAYVMANV 148* ASKEILDEAYVMASA 149* ASKEILDEAYVMASG 150*ASKEILDEAYVMASV 151* ASKEILDEAYVMTCA 152* ASKEILDEAYVMTCG 153*ASKEILDEAYVMTCV 154* ASKEILDEAYVMTIA 155* ASKEILDEAYVMTIG 156*ASKEILDEAYVMTIV 157* ASKEILDEAYVMTNA 158* ASKEILDEAYVMTNG 159*ASKEILDEAYVMTNV 160* ASKEILDEAYVMTSA 161* ASKEILDEAYVMTSG 162*ASKEILDEAYVMTSV 163* CLLVHRDLAARNV 164* CRLVHRDLAARNV 165*DDKDILDEAYVMACA 166* DDKDILDEAYVMACG 167* DDKDILDEAYVMACV 168*DDKDILDEAYVMAIA 169* DDKDILDEAYVMAIG 170* DDKDILDEAYVMAIV 171*DDKDILDEAYVMANA 172* DDKDILDEAYVMANG 173* DDKDILDEAYVMANV 174*DDKDILDEAYVMASA 175* DDKDILDEAYVMASG 176* DDKDILDEAYVMASV 177*DDKDILDEAYVMTCA 178* DDKDILDEAYVMTCG 179* DDKDILDEAYVMTCV 180*DDKDILDEAYVMTIA 181* DDKDILDEAYVMTIG 182* DDKDILDEAYVMTIV 183*DDKDILDEAYVMTNA 184* DDKDILDEAYVMTNG 185* DDKDILDEAYVMTNV 186*DDKDILDEAYVMTSA 187* DDKDILDEAYVMTSG 188* DDKDILDEAYVMTSV 189*DDKEILDEAYVMACA 190* DDKEILDEAYVMACG 191* DDKEILDEAYVMACV 192*DDKEILDEAYVMAIA 193* DDKEILDEAYVMAIG 194* DDKEILDEAYVMAIV 195*DDKEILDEAYVMANA 196* DDKEILDEAYVMANG 197* DDKEILDEAYVMANV 198*DDKEILDEAYVMASA 199* DDKEILDEAYVMASG 200* DDKEILDEAYVMASV 201*DDKEILDEAYVMTCA 202* DDKEILDEAYVMTCG 203* DDKEILDEAYVMTCV 204*DDKEILDEAYVMTIA 205* DDKEILDEAYVMTIG 206* DDKEILDEAYVMTIV 207*DDKEILDEAYVMTNA 208* DDKEILDEAYVMTNG 209* DDKEILDEAYVMTNV 210*DDKEILDEAYVMTSA 211* DDKEILDEAYVMTSG 212* DDKEILDEAYVMTSV 213*DNKDILDEAYVMACA 214* DNKDILDEAYVMACG 215* DNKDILDEAYVMACV 216*DNKDILDEAYVMAIA 217* DNKDILDEAYVMAIG 218* DNKDILDEAYVMAIV 219*DNKDILDEAYVMANA 220* DNKDILDEAYVMANG 221* DNKDILDEAYVMANV 222*DNKDILDEAYVMASA 223* DNKDILDEAYVMASG 224* DNKDILDEAYVMASV 225*DNKDILDEAYVMTCA 226* DNKDILDEAYVMTCG 227* DNKDILDEAYVMTCV 228*DNKDILDEAYVMTIA 229* DNKDILDEAYVMTIG 230* DNKDILDEAYVMTIV 231*DNKDILDEAYVMTNA 232* DNKDILDEAYVMTNG 233* DNKDILDEAYVMTNV 234*DNKDILDEAYVMTSA 235* DNKDILDEAYVMTSG 236* DNKDILDEAYVMTSV 237*DNKEILDEAYVMACA 238* DNKEILDEAYVMACG 239* DNKEILDEAYVMACV 240*DNKEILDEAYVMAIA 241* DNKEILDEAYVMAIG 242* DNKEILDEAYVMAIV 243*DNKEILDEAYVMANA 244* DNKEILDEAYVMANG 245* DNKEILDEAYVMANV 246*DNKEILDEAYVMASA 247* DNKEILDEAYVMASG 248* DNKEILDEAYVMASV 249*DNKEILDEAYVMTCA 250* DNKEILDEAYVMTCG 251* DNKEILDEAYVMTCV 252*DNKEILDEAYVMTIA 253* DNKEILDEAYVMTIG 254* DNKEILDEAYVMTIV 255*DNKEILDEAYVMTNA 256* DNKEILDEAYVMTNG 257* DNKEILDEAYVMTNV 258*DNKEILDEAYVMTSA 259* DNKEILDEAYVMTSG 260* DNKEILDEAYVMTSV 261*DSKDILDEAYVMACA 262* DSKDILDEAYVMACG 263* DSKDILDEAYVMACV 264*DSKDILDEAYVMAIA 265* DSKDILDEAYVMAIG 266* DSKDILDEAYVMAIV 267*DSKDILDEAYVMANA 268* DSKDILDEAYVMANG 269* DSKDILDEAYVMANV 270*DSKDILDEAYVMASA 271* DSKDILDEAYVMASG 272* DSKDILDEAYVMASV 273*DSKDILDEAYVMTCA 274* DSKDILDEAYVMTCG 275* DSKDILDEAYVMTCV 276*DSKDILDEAYVMTIA 277* DSKDILDEAYVMTIG 278* DSKDILDEAYVMTIV 279*DSKDILDEAYVMTNA 280* DSKDILDEAYVMTNG 281* DSKDILDEAYVMTNV 282*DSKDILDEAYVMTSA 283* DSKDILDEAYVMTSG 284* DSKDILDEAYVMTSV 285*DSKEILDEAYVMACA 286* DSKEILDEAYVMACG 287* DSKEILDEAYVMACV 288*DSKEILDEAYVMAIA 289* DSKEILDEAYVMAIG 290* DSKEILDEAYVMAIV 291*DSKEILDEAYVMANA 292* DSKEILDEAYVMANG 293* DSKEILDEAYVMANV 294*DSKEILDEAYVMASA 295* DSKEILDEAYVMASG 296* DSKEILDEAYVMASV 297*DSKEILDEAYVMTCA 298* DSKEILDEAYVMTCG 299* DSKEILDEAYVMTCV 300*DSKEILDEAYVMTIA 301* DSKEILDEAYVMTIG 302* DSKEILDEAYVMTIV 303*DSKEILDEAYVMTNA 304* DSKEILDEAYVMTNG 305* DSKEILDEAYVMTNV 306*DSKEILDEAYVMTSA 307* DSKEILDEAYVMTSG 308* DSKEILDEAYVMTSV 309 HYQDPHSTAVGNPEY 310* KVKIPVAIAPSPKA 311* KVKIPVAIATSPKA 312*KVKIPVAIEAPSPKA 313* KVKIPVAIEATSPKA 314* KVKIPVAIEEAPSPKA 315*KVKIPVAIEEATSPKA 316* KVKIPVAIEEPSPKA 317* KVKIPVAIEETSPKA 318*KVKIPVAIEPSPKA 319* KVKIPVAIERAPSPKA 320* KVKIPVAIERATSPKA 321*KVKIPVAIEREAPSPKA 322* KVKIPVAIEREATSPKA 323* KVKIPVAIEREPSPKA 324*KVKIPVAIERETSPKA 325* KVKIPVAIERPSPKA 326* KVKIPVAIERTSPKA 327*KVKIPVAIETSPKA 328* KVKIPVAIKAPSPKA 329* KVKIPVAIKATSPKA 330*KVKIPVAIKEAPSPKA 331* KVKIPVAIKEATSPKA 332* KVKIPVAIKEEAPSPKA 333*KVKIPVAIKEEATSPKA 334* KVKIPVAIKEEPSPKA 335* KVKIPVAIKEETSPKA 336*KVKIPVAIKEPSPKA 337* KVKIPVAIKERAPSPKA 338* KVKIPVAIKERATSPKA 339*KVKIPVAIKEREAPSPKA 340* KVKIPVAIKEREATSPKA 341* KVKIPVAIKEREPSPKA 342*KVKIPVAIKERETSPKA 343* KVKIPVAIKERPSPKA 344* KVKIPVAIKERTSPKA 345*KVKIPVAIKETSPKA 346* KVKIPVAIKPSPKA 347* KVKIPVAIKRAPSPKA 348*KVKIPVAIKRATSPKA 349* KVKIPVAIKREAPSPKA 350* KVKIPVAIKREATSPKA 351*KVKIPVAIKREPSPKA 352* KVKIPVAIKRETSPKA 353* KVKIPVAIKRPSPKA 354*KVKIPVAIKRTSPKA 355* KVKIPVAIKTSPKA 356* KVKIPVAIPSPKA 357*KVKIPVAIRAPSPKA 358* KVKIPVAIRATSPKA 359* KVKIPVAIREAPSPKA 360*KVKIPVAIREATSPKA 361* KVKIPVAIREP SPKA 362* KVKIPVAIRETSPKA 363*KVKIPVAIRPSPKA 364* KVKIPVAIRTSPKA 365* KVKIPVAITSPKA 366* LREEILDEAYVMA367* LREPILDEAYVMA 368* PEGEKAKIPVAIKELREA 369* PEGEKAKIPVAIRELREA 370 PEGEKVKIPVAIKELREA 371* PEGEKVKIPVAIRELREA 372* RLLVHRDLAARNV 373 RRLVHRDLAARNV 374* SDKDILDEAYVMACA 375* SDKDILDEAYVMACG 376*SDKDILDEAYVMACV 377* SDKDILDEAYVMAIA 378* SDKDILDEAYVMAIG 379*SDKDILDEAYVMAIV 380* SDKDILDEAYVMANA 381* SDKDILDEAYVMANG 382*SDKDILDEAYVMANV 383* SDKDILDEAYVMASA 384* SDKDILDEAYVMASG 385*SDKDILDEAYVMASV 386* SDKDILDEAYVMTCA 387* SDKDILDEAYVMTCG 388*SDKDILDEAYVMTCV 389* SDKDILDEAYVMTIA 390* SDKDILDEAYVMTIG 391*SDKDILDEAYVMTIV 392* SDKDILDEAYVMTNA 393* SDKDILDEAYVMTNG 394*SDKDILDEAYVMTNV 395* SDKDILDEAYVMTSA 396* SDKDILDEAYVMTSG 397*SDKDILDEAYVMTSV 398* SDKEILDEAYVMACA 399* SDKEILDEAYVMACG 400*SDKEILDEAYVMACV 401* SDKEILDEAYVMAIA 402* SDKEILDEAYVMAIG 403*SDKEILDEAYVMAIV 404* SDKEILDEAYVMANA 405* SDKEILDEAYVMANG 406*SDKEILDEAYVMANV 407* SDKEILDEAYVMASA 408* SDKEILDEAYVMASG 409*SDKEILDEAYVMASV 410* SDKEILDEAYVMTCA 411* SDKEILDEAYVMTCG 412*SDKEILDEAYVMTCV 413* SDKEILDEAYVMTIA 414* SDKEILDEAYVMTIG 415*SDKEILDEAYVMTIV 416* SDKEILDEAYVMTNA 417* SDKEILDEAYVMTNG 418*SDKEILDEAYVMTNV 419* SDKEILDEAYVMTSA 420* SDKEILDEAYVMTSG 421*SDKEILDEAYVMTSV 422* SNKDILDEAYVMACA 423* SNKDILDEAYVMACG 424*SNKDILDEAYVMACV 425* SNKDILDEAYVMAIA 426* SNKDILDEAYVMAIG 427*SNKDILDEAYVMAIV 428* SNKDILDEAYVMANA 429* SNKDILDEAYVMANG 430*SNKDILDEAYVMANV 431* SNKDILDEAYVMASA 432* SNKDILDEAYVMASG 433*SNKDILDEAYVMASV 434* SNKDILDEAYVMTCA 435* SNKDILDEAYVMTCG 436*SNKDILDEAYVMTCV 437* SNKDILDEAYVMTIA 438* SNKDILDEAYVMTIG 439*SNKDILDEAYVMTIV 440* SNKDILDEAYVMTNA 441* SNKDILDEAYVMTNG 442*SNKDILDEAYVMTNV 443* SNKDILDEAYVMTSA 444* SNKDILDEAYVMTSG 445*SNKDILDEAYVMTSV 446* SNKEILDEAYVMACA 447* SNKEILDEAYVMACG 448*SNKEILDEAYVMACV 449* SNKEILDEAYVMAIA 450* SNKEILDEAYVMAIG 451*SNKEILDEAYVMAIV 452* SNKEILDEAYVMANA 453* SNKEILDEAYVMANG 454*SNKEILDEAYVMANV 455* SNKEILDEAYVMASA 456* SNKEILDEAYVMASG 457*SNKEILDEAYVMASV 458* SNKEILDEAYVMTCA 459* SNKEILDEAYVMTCG 460*SNKEILDEAYVMTCV 461* SNKEILDEAYVMTIA 462* SNKEILDEAYVMTIG 463*SNKEILDEAYVMTIV 464* SNKEILDEAYVMTNA 465* SNKEILDEAYVMTNG 466*SNKEILDEAYVMTNV 467* SNKEILDEAYVMTSA 468* SNKEILDEAYVMTSG 469*SNKEILDEAYVMTSV 470* SSKDILDEAYVMACA 471* SSKDILDEAYVMACG 472*SSKDILDEAYVMACV 473* SSKDILDEAYVMAIA 474* SSKDILDEAYVMAIG 475*SSKDILDEAYVMAIV 476* SSKDILDEAYVMANA 477* SSKDILDEAYVMANG 478*SSKDILDEAYVMANV 479* SSKDILDEAYVMASA 480* SSKDILDEAYVMASG 481*SSKDILDEAYVMASV 482* SSKDILDEAYVMTCA 483* SSKDILDEAYVMTCG 484*SSKDILDEAYVMTCV 485* SSKDILDEAYVMTIA 486* SSKDILDEAYVMTIG 487*SSKDILDEAYVMTIV 488* SSKDILDEAYVMTNA 489* SSKDILDEAYVMTNG 490*SSKDILDEAYVMTNV 491* SSKDILDEAYVMTSA 492* SSKDILDEAYVMTSG 493*SSKDILDEAYVMTSV 494* SSKEILDEAYVMACA 495* SSKEILDEAYVMACG 496*SSKEILDEAYVMACV 497* SSKEILDEAYVMAIA 498* SSKEILDEAYVMAIG 499*SSKEILDEAYVMAIV 500* SSKEILDEAYVMANA 501* SSKEILDEAYVMANG 502*SSKEILDEAYVMANV 503* SSKEILDEAYVMASA 504* SSKEILDEAYVMASG 505*SSKEILDEAYVMASV 506* SKEILDEAYVMTCA 507* SSKEILDEAYVMTCG 508*SSKEILDEAYVMTCV 509* SSKEILDEAYVMTIA 510* SSKEILDEAYVMTIG 511*SSKEILDEAYVMTIV 512* SSKEILDEAYVMTNA 513* SSKEILDEAYVMTNG 514*SSKEILDEAYVMTNV 515* SSKEILDEAYVMTSA 516* SSKEILDEAYVMTSG 517*SSKEILDEAYVMTSV 518* AVVMASVDNPHVCR 519  AYVMASVDNPHVCR 520 CTGPGLEGCPTNG 521  DEAYVMASVDNPHVCRLLG 522* ILKETEFKKIFVLGPGAFGT 523*ILKETEFKKIFVLGSGAFGT 524* ILKETEFKKIKVLGPGAFGT 525  ILKETEFKKIKVLGSGAFGT526* ILKETEFKKLFVLGPGAFGT 527* ILKETEFKKLFVLGSGAFGT 528*ILKETEFKKLKVLGPGAFGT 529* ILKETEFKKLKVLGSGAFGT 530* ILKETELKKIFVLGPGAFGT531* ILKETELKKIFVLGSGAFGT 532* ILKETELKKIKVLGPGAFGT 533*ILKETELKKIKVLGSGAFGT 534* ILKETELKKLFVLGPGAFGT 535* ILKETELKKLFVLGSGAFGT536* ILKETELKKLKVLGPGAFGT 537* ILKETELKKLKVLGSGAFGT 538*ILMETEFKKIFVLGPGAFGT 539* ILMETEFKKIFVLGSGAFGT 540* ILMETEFKKIKVLGPGAFGT541* ILMETEFKKIKVLGSGAFGT 542* ILMETEFKKLFVLGPGAFGT 543*ILMETEFKKLFVLGSGAFGT 544* ILMETEFKKLKVLGPGAFGT 545* ILMETEFKKLKVLGSGAFGT546* ILMETELKKIFVLGPGAFGT 547* ILMETELKKIFVLGSGAFGT 548*ILMETELKKIKVLGPGAFGT 549* ILMETELKKIKVLGSGAFGT 550* ILMETELKKLFVLGPGAFGT551* ILMETELKKLFVLGSGAFGT 552* ILMETELKKLKVLGPGAFGT 553*ILMETELKKLKVLGSGAFGT 554* KGNYVVTDHGSCVRA 555* KVKIPVAIKAPKA 556 KVKIPVAIKELREATSPKA 557* KVKIPVAIKSPKA 558  LLRILKETEFKKI 559*LLRILKETESKKI 560* MNYLEDRLLVHRD 561  MNYLEDRRLVHRD 562*RLLQERELLEPLTPSGEAPNQAFLR 563* RLLQERELLEPLTPSGEAPNQALLR 564*RLLQERELLEPLTPSGEAPNQAPLR 565* RLLQERELVEPLTPSGEAPNQAFLR 566 RLLQERELVEPLTPSGEAPNQALLR 567* RLLQERELVEPLTPSGEAPNQAPLR 568*TLKETEFKKIFVLGPGAFGT 569* TLKETEFKKIFVLGSGAFGT 570* TLKETEFKKIKVLGPGAFGT571* TLKETEFKKIKVLGSGAFGT 572* TLKETEFKKLFVLGPGAFGT 573*TLKETEFKKLFVLGSGAFGT 574* TLKETEFKKLKVLGPGAFGT 575* TLKETEFKKLKVLGSGAFGT576* TLKETELKKIFVLGPGAFGT 577* TLKETELKKIFVLGSGAFGT 578*TLKETELKKIKVLGPGAFGT 579* TLKETELKKIKVLGSGAFGT 580* TLKETELKKLFVLGPGAFGT581* TLKETELKKLFVLGSGAFGT 582* TLKETELKKLKVLGPGAFGT 583*TLKETELKKLKVLGSGAFGT 584* TLMETEFKKIFVLGPGAFGT 585* TLMETEFKKIFVLGSGAFGT586* TLMETEFKKIKVLGPGAFGT 587* TLMETEFKKIKVLGSGAFGT 588*TLMETEFKKLFVLGPGAFGT 589* TLMETEFKKLFVLGSGAFGT 590* TLMETEFKKLKVLGPGAFGT591* TLMETEFKKLKVLGSGAFGT 592* TLMETELKKIFVLGPGAFGT 593*TLMETELKKIFVLGSGAFGT 594* TLMETELKKIKVLGPGAFGT 595* TLMETELKKIKVLGSGAFGT596* TLMETELKKLFVLGPGAFGT 597* TLMETELKKLFVLGSGAFGT 598*TLMETELKKLKVLGPGAFGT 599* TLMETELKKLKVLGSGAFGT 600  VKITDFGLAKLLGA 601*VKITDFGRAKLLGA 602  YLEDRRLVHRDLA 603  KVKIPVAIKELREATSPKA 604*KVKIPVAIKAPKA 605* KVKIPVAIKAPTS 606* KVKIPVAIKDPKA 607* KVKIPVAIKELKA608* KVKIPVAIKEPKA 609* KVKIPVAIKEQKA 610* KVKIPVAIKESKA 611*KVKIPVAIKEVPK 612* KVKIPVAIKIPKA 613* KVKIPVAIKSPKA 614* KVKIPVAIKTPKA615* KIPVAIKEASPKA 616* KIPVAIKEFSPKA 617* KIPVAIKENSPKA 618*KIPVAIKVASPKA 619* KIPVAIKVPSPKA

Experiments Peptide Arrays

Peptide arrays were created by PepStar™ (JPT Peptide Technologies GmbH,Berlin, Germany) peptide microarray platform to generate customizedpeptide microarrays on glass slides for biomarker discovery,immunomonitoring and detection and validation of protein interactions.Peptides are immobilized on glass slides via a flexible linker.Chemoselective coupling generates microarrays of directed and covalentlyattached peptides.

3661 specified native peptides with 500 scrambled control sequencescovering the sequences of EGF-receptor, Arachidonate 15-lipoxygenase B(LX15B) and p53 and variants thereof were synthesized and arraysprinted. A serum dilution 1:200 was used for the detection of EGFRbinding in NSCLC patient samples. Incubation of microarrays wasperformed in an automated Hybridization Station HS4800 (Tecan) at 30° C.After washing, bound immunoglobine G (IgG) was detected withCy5—labeled-anti-human secondary antibody (JIR, 0.1 μg/ml end-conc. inassay). Fluorescence was read using a Microarray Scanner GenePix withAutoloader 4200AL (Molecular Devices). Signal intensity is displayed asrelative fluorescence units.

The following tables detail the wash and incubation conditions:

TABLE 5 experimental conditions Blocking Solution: SmartBlock BlockingBuffer Diluent for Serum and 2nd AB: SuperBlock T20 Blocking Buffer WashBuffer 1:   1x TBS + 0.1% Tween 20 Wash Buffer 2: 0.1x SSC + 0.05% Tween20

TABLE 6 experimental conditions Step Procedure Parameters Buffer  1 Wash1x   1x TBS-0, 1% Tween20  2 Probe injection 1 Blocking Solution  3Hybridization 1 h, 30° C.  4 Wash 2x   1x TBS-0, 1% Tween20  5 Probeinjection 2 1:200 Patient Serum  6 Hybridization 2 h, 30° C.  7 Wash 3x  1x TBS-0, 1% Tween20  8 Probe injection 3 Cy5-anti-human SecondaryAntibody  9 Hybridization 45 min., 30° C. 10 Wash 2x   1x TBS-0, 1%Tween20 11 Wash 3x 0.1x SSC-0.05% Tween20 12 Slide drying 20 sec 13 Wash3x 0.1x SSC-0.05% Tween20 14 Slide drying 5 min.

Samples

Samples were selected from patients belonging to the TASK study. TASKwas a 200-patient randomized, open label, Phase II study of Tarceva® incombination with Avastin® (bevazizumab) compared to standardchemotherapy regimens (gemcitabine plus cisplatin or paclitaxel pluscarboplatin) plus Avastin® in first-line NSCLC patients. Furtherenrollment on this study was halt after data from a pre-planned interimanalysis of the first 120 patients. Occurrence of rash was recorded asadverse event. Biopsies from all patients were tested for the occurrenceof EGFR mutations and the mutation status has been recorded.

For the peptide array study, a total of 49 patients from both arms (24Avastin® and Tarceva® (A+T), 25 Avastin® and chemotherapy (A+C)) havebeen used. In the patients chosen for the A+T arm, rash occurred in 16patients. In comparison, only 3 patients in the A+C arm developed rash.This corresponds to the expected frequency of rash, as rash is inducedby erlotinib, but only to a very limited extend by chemotherapy.

Clinical data accessible included outcome data, e.g. response overallsurvival and progression free survival as well as the rash grade and themutation status of EGFR as measured from a pre-treatment biopsy.

Statistical Analysis Global Test

The following approach to model the data was applied:

-   -   Descriptive statistics (using appropriate single variable) tests        to identify covariates as candidates for a linear regression        model describing the performance parameter (PFS or OS).    -   Identification of the best model for efficacy parameters        (survival analysis)    -   Addition of antibody titer and its interaction with treatment to        the model and comparison of the performance with the new        variables        With a general model selected, tests on antibody titers within        groups of covariates of interest were conducted:    -   Proportional hazard model by EGFR mutation (EGFRM), Thoracic        Radiotherapy (TRT), rash of efficacy parameters vs. antibody        abundance (two sample groups—low/high abundance—divided by        median)    -   U-tests within wild type (WT+) in A+T arm of antibody titer vs.        responders/non-responders    -   χ2 tests by EGFRM and TRT of responses (weeks 6, 12) vs.        antibody abundance

Finally, various very similar peptide sequences that yield highlycorrelated antibody titers were used. For most of the reported peptidesequences, consensus sequence from a listing of all peptides with veryhigh sequence similarity (Smith-Waterman alignment similarity score >50,using a PAM30 substitution matrix and a gap penalty of 1) was selectedand antibody titers were correlated to the target titer (Spearman Rankcorrelation >0.75).

1. A method of diagnosis of non-small cell lung cancer in a humansubject comprising: measuring in a blood sample of the human subject alevel of an autoantibody selected from the group of autoantibodiesrecognizing mutated human EGFR, wherein an increased level of saidautoantibody selected from the group of autoantibodies recognizingmutated human EGFR in the blood sample of the human subject compared toa level of said autoantibody representative for a human subject of ahealthy population is indicative for non-small cell lung cancer.
 2. Themethod according to claim 1, wherein the autoantibody recognizes amutated EGFR peptide is selected from the group consisting of Seq. Id.No. 554, Seq. Id. No. 555, Seq. Id. No. 556, Seq. Id. No. 557, Seq. Id.No. 558, Seq. Id. No. 559, and Seq. Id. No.
 560. 3. The method accordingto any of claims 1-2, wherein the autoantibody recognizes a mutated EGFRpeptide is selected from the group consisting of Seq. Id. No. 1, Seq.Id. No. 2, Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No.7, Seq. Id. No. 15, Seq. Id. No. 16, Seq. Id. No. 17, Seq. Id. No. 18,Seq. Id. No. 19, Seq. Id. No. 20, Seq. Id. No. 21, Seq. Id. No. 22, Seq.Id. No. 23, Seq. Id. No. 24, Seq. Id. No. 25, Seq. Id. No. 26, Seq. Id.No. 27, Seq. Id. No. 28, Seq. Id. No. 29, Seq. Id. No. 30, Seq. Id. No.31, Seq. Id. No. 32, Seq. Id. No. 33, Seq. Id. No. 34, Seq. Id. No. 35,Seq. Id. No. 36, Seq. Id. No. 37, Seq. Id. No. 38, Seq. Id. No. 39, Seq.Id. No. 40, Seq. Id. No. 41, Seq. Id. No. 42, Seq. Id. No. 43, Seq. Id.No. 44, Seq. Id. No. 45, Seq. Id. No. 46, Seq. Id. No. 47, Seq. Id. No.48, Seq. Id. No. 49, Seq. Id. No. 50, Seq. Id. No. 51, Seq. Id. No. 52,Seq. Id. No. 53, Seq. Id. No. 54, Seq. Id. No. 55, Seq. Id. No. 56, Seq.Id. No. 57, Seq. Id. No. 58, Seq. Id. No. 59, Seq. Id. No. 60, Seq. Id.No. 61, Seq. Id. No. 62, Seq. Id. No. 63, Seq. Id. No. 65, Seq. Id. No.66, Seq. Id. No. 67, Seq. Id. No. 68, Seq. Id. No. 69, Seq. Id. No. 70,Seq. Id. No. 71, Seq. Id. No. 72, Seq. Id. No. 73, Seq. Id. No. 74, Seq.Id. No. 75, Seq. Id. No. 76, Seq. Id. No. 77, Seq. Id. No. 78, Seq. Id.No. 79, Seq. Id. No. 80, Seq. Id. No. 81, Seq. Id. No. 82, Seq. Id. No.83, Seq. Id. No. 84, Seq. Id. No. 85, Seq. Id. No. 86, Seq. Id. No. 87,Seq. Id. No. 88, Seq. Id. No. 89, Seq. Id. No. 90, Seq. Id. No. 91, Seq.Id. No. 92, Seq. Id. No. 93, Seq. Id. No. 94, Seq. Id. No. 95, Seq. Id.No. 96, Seq. Id. No. 97, Seq. Id. No. 98, Seq. Id. No. 99, Seq. Id. No.100, Seq. Id. No. 101, Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No.105, Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No.109, Seq. Id. No. 110, Seq. Id. No. 111, Seq. Id. No. 112, Seq. Id. No.113, Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No.117, Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No.121, Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No.125, Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No.129, Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No.133, Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No.137, Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No.141, Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No.145, Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No.149, Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No.153, Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No.157, Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No.161, Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No.165, Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No.169, Seq. Id. No. 170, Seq. Id. No. 171, Seq. Id. No. 172, Seq. Id. No.173, Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No.177, Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No.181, Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No.185, Seq. Id. No. 186, Seq. Id. No. 187, Seq. Id. No. 188, Seq. Id. No.189, Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No.193, Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No.197, Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id. No. 200, Seq. Id. No.201, Seq. Id. No. 202, Seq. Id. No. 203, Seq. Id. No. 204, Seq. Id. No.205, Seq. Id. No. 206, Seq. Id. No. 207, Seq. Id. No. 208, Seq. Id. No.209, Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No.213, Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No.217, Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id. No. 220, Seq. Id. No.221, Seq. Id. No. 222, Seq. Id. No. 223, Seq. Id. No. 224, Seq. Id. No.225, Seq. Id. No. 226, Seq. Id. No. 227, Seq. Id. No. 228, Seq. Id. No.229, Seq. Id. No. 230, Seq. Id. No. 231, Seq. Id. No. 232, Seq. Id. No.233, Seq. Id. No. 234, Seq. Id. No. 235, Seq. Id. No. 236, Seq. Id. No.237, Seq. Id. No. 238, Seq. Id. No. 239, Seq. Id. No. 240, Seq. Id. No.241, Seq. Id. No. 242, Seq. Id. No. 243, Seq. Id. No. 244, Seq. Id. No.245, Seq. Id. No. 246, Seq. Id. No. 247, Seq. Id. No. 248, Seq. Id. No.249, Seq. Id. No. 250, Seq. Id. No. 251, Seq. Id. No. 252, Seq. Id. No.253, Seq. Id. No. 254, Seq. Id. No. 255, Seq. Id. No. 256, Seq. Id. No.257, Seq. Id. No. 258, Seq. Id. No. 259, Seq. Id. No. 260, Seq. Id. No.261, Seq. Id. No. 262, Seq. Id. No. 263, Seq. Id. No. 264, Seq. Id. No.265, Seq. Id. No. 266, Seq. Id. No. 267, Seq. Id. No. 268, Seq. Id. No.269, Seq. Id. No. 270, Seq. Id. No. 271, Seq. Id. No. 272, Seq. Id. No.273, Seq. Id. No. 274, Seq. Id. No. 275, Seq. Id. No. 276, Seq. Id. No.277, Seq. Id. No. 278, Seq. Id. No. 279, Seq. Id. No. 280, Seq. Id. No.281, Seq. Id. No. 282, Seq. Id. No. 283, Seq. Id. No. 284, Seq. Id. No.285, Seq. Id. No. 286, Seq. Id. No. 287, Seq. Id. No. 288, Seq. Id. No.289, Seq. Id. No. 290, Seq. Id. No. 291, Seq. Id. No. 292, Seq. Id. No.293, Seq. Id. No. 294, Seq. Id. No. 295, Seq. Id. No. 296, Seq. Id. No.297, Seq. Id. No. 298, Seq. Id. No. 299, Seq. Id. No. 300, Seq. Id. No.301, Seq. Id. No. 302, Seq. Id. No. 303, Seq. Id. No. 304, Seq. Id. No.305, Seq. Id. No. 306, Seq. Id. No. 307, Seq. Id. No. 308, Seq. Id. No.310, Seq. Id. No. 311, Seq. Id. No. 312, Seq. Id. No. 313, Seq. Id. No.314, Seq. Id. No. 315, Seq. Id. No. 316, Seq. Id. No. 317, Seq. Id. No.318, Seq. Id. No. 319, Seq. Id. No. 320, Seq. Id. No. 321, Seq. Id. No.322, Seq. Id. No. 323, Seq. Id. No. 324, Seq. Id. No. 325, Seq. Id. No.326, Seq. Id. No. 327, Seq. Id. No. 328, Seq. Id. No. 329, Seq. Id. No.330, Seq. Id. No. 331, Seq. Id. No. 332, Seq. Id. No. 333, Seq. Id. No.334, Seq. Id. No. 335, Seq. Id. No. 336, Seq. Id. No. 337, Seq. Id. No.338, Seq. Id. No. 339, Seq. Id. No. 340, Seq. Id. No. 341, Seq. Id. No.342, Seq. Id. No. 343, Seq. Id. No. 344, Seq. Id. No. 345, Seq. Id. No.346, Seq. Id. No. 347, Seq. Id. No. 348, Seq. Id. No. 349, Seq. Id. No.350, Seq. Id. No. 351, Seq. Id. No. 352, Seq. Id. No. 353, Seq. Id. No.354, Seq. Id. No. 355, Seq. Id. No. 356, Seq. Id. No. 357, Seq. Id. No.358, Seq. Id. No. 359, Seq. Id. No. 360, Seq. Id. No. 361, Seq. Id. No.362, Seq. Id. No. 363, Seq. Id. No. 364, Seq. Id. No. 365, Seq. Id. No.366, Seq. Id. No. 367, Seq. Id. No. 368, Seq. Id. No. 369, Seq. Id. No.371, Seq. Id. No. 372, Seq. Id. No. 374, Seq. Id. No. 375, Seq. Id. No.376, Seq. Id. No. 377, Seq. Id. No. 378, Seq. Id. No. 379, Seq. Id. No.380, Seq. Id. No. 381, Seq. Id. No. 382, Seq. Id. No. 383, Seq. Id. No.384, Seq. Id. No. 385, Seq. Id. No. 386, Seq. Id. No. 387, Seq. Id. No.388, Seq. Id. No. 389, Seq. Id. No. 390, Seq. Id. No. 391, Seq. Id. No.392, Seq. Id. No. 393, Seq. Id. No. 394, Seq. Id. No. 395, Seq. Id. No.396, Seq. Id. No. 397, Seq. Id. No. 398, Seq. Id. No. 399, Seq. Id. No.400, Seq. Id. No. 401, Seq. Id. No. 402, Seq. Id. No. 403, Seq. Id. No.404, Seq. Id. No. 405, Seq. Id. No. 406, Seq. Id. No. 407, Seq. Id. No.408, Seq. Id. No. 409, Seq. Id. No. 410, Seq. Id. No. 411, Seq. Id. No.412, Seq. Id. No. 413, Seq. Id. No. 414, Seq. Id. No. 415, Seq. Id. No.416, Seq. Id. No. 417, Seq. Id. No. 418, Seq. Id. No. 419, Seq. Id. No.420, Seq. Id. No. 421, Seq. Id. No. 422, Seq. Id. No. 423, Seq. Id. No.424, Seq. Id. No. 425, Seq. Id. No. 426, Seq. Id. No. 427, Seq. Id. No.428, Seq. Id. No. 429, Seq. Id. No. 430, Seq. Id. No. 431, Seq. Id. No.432, Seq. Id. No. 433, Seq. Id. No. 434, Seq. Id. No. 435, Seq. Id. No.436, Seq. Id. No. 437, Seq. Id. No. 438, Seq. Id. No. 439, Seq. Id. No.440, Seq. Id. No. 441, Seq. Id. No. 442, Seq. Id. No. 443, Seq. Id. No.444, Seq. Id. No. 445, Seq. Id. No. 446, Seq. Id. No. 447, Seq. Id. No.448, Seq. Id. No. 449, Seq. Id. No. 450, Seq. Id. No. 451, Seq. Id. No.452, Seq. Id. No. 453, Seq. Id. No. 454, Seq. Id. No. 455, Seq. Id. No.456, Seq. Id. No. 457, Seq. Id. No. 458, Seq. Id. No. 459, Seq. Id. No.460, Seq. Id. No. 461, Seq. Id. No. 462, Seq. Id. No. 463, Seq. Id. No.464, Seq. Id. No. 465, Seq. Id. No. 466, Seq. Id. No. 467, Seq. Id. No.468, Seq. Id. No. 469, Seq. Id. No. 470, Seq. Id. No. 471, Seq. Id. No.472, Seq. Id. No. 473, Seq. Id. No. 474, Seq. Id. No. 475, Seq. Id. No.476, Seq. Id. No. 477, Seq. Id. No. 478, Seq. Id. No. 479, Seq. Id. No.480, Seq. Id. No. 481, Seq. Id. No. 482, Seq. Id. No. 483, Seq. Id. No.484, Seq. Id. No. 485, Seq. Id. No. 486, Seq. Id. No. 487, Seq. Id. No.488, Seq. Id. No. 489, Seq. Id. No. 490, Seq. Id. No. 491, Seq. Id. No.492, Seq. Id. No. 493, Seq. Id. No. 494, Seq. Id. No. 495, Seq. Id. No.496, Seq. Id. No. 497, Seq. Id. No. 498, Seq. Id. No. 499, Seq. Id. No.500, Seq. Id. No. 501, Seq. Id. No. 502, Seq. Id. No. 503, Seq. Id. No.504, Seq. Id. No. 505, Seq. Id. No. 506, Seq. Id. No. 507, Seq. Id. No.508, Seq. Id. No. 509, Seq. Id. No. 510, Seq. Id. No. 511, Seq. Id. No.512, Seq. Id. No. 513, Seq. Id. No. 514, Seq. Id. No. 515, Seq. Id. No.516, Seq. Id. No. 517, Seq. Id. No. 518, Seq. Id. No. 522, Seq. Id. No.523, Seq. Id. No. 524, Seq. Id. No. 526, Seq. Id. No. 527, Seq. Id. No.528, Seq. Id. No. 529, Seq. Id. No. 530, Seq. Id. No. 531, Seq. Id. No.532, Seq. Id. No. 533, Seq. Id. No. 534, Seq. Id. No. 535, Seq. Id. No.536, Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No. 539, Seq. Id. No.540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No. 543, Seq. Id. No.544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No. 547, Seq. Id. No.548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No. 551, Seq. Id. No.552, Seq. Id. No. 553, Seq. Id. No. 554, Seq. Id. No. 555, Seq. Id. No.557, Seq. Id. No. 559, Seq. Id. No. 560, Seq. Id. No. 562, Seq. Id. No.563, Seq. Id. No. 564, Seq. Id. No. 565, Seq. Id. No. 567, Seq. Id. No.568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No. 571, Seq. Id. No.572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No. 575, Seq. Id. No.576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No. 579, Seq. Id. No.580, Seq. Id. No. 581, Seq. Id. No. 582, Seq. Id. No. 583, Seq. Id. No.584, Seq. Id. No. 585, Seq. Id. No. 586, Seq. Id. No. 587, Seq. Id. No.588, Seq. Id. No. 589, Seq. Id. No. 590, Seq. Id. No. 591, Seq. Id. No.592, Seq. Id. No. 593, Seq. Id. No. 594, Seq. Id. No. 595, Seq. Id. No.596, Seq. Id. No. 597, Seq. Id. No. 598, Seq. Id. No. 599, Seq. Id. No.601, Seq. Id. No. 604, Seq. Id. No. 605, Seq. Id. No. 606, Seq. Id. No.607, Seq. Id. No. 608, Seq. Id. No. 609, Seq. Id. No. 610, Seq. Id. No.611, Seq. Id. No. 612, Seq. Id. No. 613, Seq. Id. No. 614, Seq. Id. No.615, Seq. Id. No. 616, Seq. Id. No. 617, Seq. Id. No. 618 and Seq. Id.No.
 619. 4. The method according to any of claims 1-3, wherein theautoantibody recognizes a mutated EGFR peptide that is selected from thegroups consisting of Seq. Id. No. 1, Seq. Id. No. 2, Seq. Id. No. 4,Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7 and Seq. Id. No.
 15. 5.The method according to any of claims 1-3, wherein the autoantibodyrecognizes a mutated EGFR peptide that is selected from the groupconsisting of Seq. Id. No. 16, Seq. Id. No. 17, Seq. Id. No. 18, Seq.Id. No. 19, Seq. Id. No. 20, Seq. Id. No. 21, Seq. Id. No. 22, Seq. Id.No. 23, Seq. Id. No. 24, Seq. Id. No. 25, Seq. Id. No. 26, Seq. Id. No.27, Seq. Id. No. 28, Seq. Id. No. 29, Seq. Id. No. 30, Seq. Id. No. 31,Seq. Id. No. 32, Seq. Id. No. 33, Seq. Id. No. 34, Seq. Id. No. 35, Seq.Id. No. 36, Seq. Id. No. 37, Seq. Id. No. 38, Seq. Id. No. 39, Seq. Id.No. 40, Seq. Id. No. 41, Seq. Id. No. 42, Seq. Id. No. 43, Seq. Id. No.44, Seq. Id. No. 45, Seq. Id. No. 46, Seq. Id. No. 47, Seq. Id. No. 48,Seq. Id. No. 49, Seq. Id. No. 50, Seq. Id. No. 51, Seq. Id. No. 52, Seq.Id. No. 53, Seq. Id. No. 54, Seq. Id. No. 55, Seq. Id. No. 56, Seq. Id.No. 57, Seq. Id. No. 58, Seq. Id. No. 59, Seq. Id. No. 60, Seq. Id. No.61, Seq. Id. No. 62, Seq. Id. No. 63, Seq. Id. No. 65, Seq. Id. No. 66,Seq. Id. No. 67, Seq. Id. No. 68, Seq. Id. No. 69, Seq. Id. No. 70, Seq.Id. No. 71, Seq. Id. No. 72, Seq. Id. No. 73, Seq. Id. No. 74, Seq. Id.No. 75, Seq. Id. No. 76, Seq. Id. No. 77, Seq. Id. No. 78, Seq. Id. No.79, Seq. Id. No. 80, Seq. Id. No. 81, Seq. Id. No. 82, Seq. Id. No. 83,Seq. Id. No. 84, Seq. Id. No. 85, Seq. Id. No. 86, Seq. Id. No. 87, Seq.Id. No. 88, Seq. Id. No. 89, Seq. Id. No. 90, Seq. Id. No. 91, Seq. Id.No. 92, Seq. Id. No. 93, Seq. Id. No. 94, Seq. Id. No. 95, Seq. Id. No.96, Seq. Id. No. 97, Seq. Id. No. 98, Seq. Id. No. 99, Seq. Id. No. 100,Seq. Id. No. 101, Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No. 105,Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No. 109,Seq. Id. No. 110, Seq. Id. No. 111, Seq. Id. No. 112, Seq. Id. No. 113,Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No. 117,Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No. 121,Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No. 125,Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No. 129,Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No. 133,Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No. 137,Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No. 141,Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No. 145,Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No. 149,Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No. 153,Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No. 157,Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No. 161,Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No. 165,Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No. 169,Seq. Id. No. 170, Seq. Id. No. 171, Seq. Id. No. 172, Seq. Id. No. 173,Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No. 177,Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No. 181,Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No. 185,Seq. Id. No. 186, Seq. Id. No. 187, Seq. Id. No. 188, Seq. Id. No. 189,Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No. 193,Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No. 197,Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id. No. 200, Seq. Id. No. 201,Seq. Id. No. 202, Seq. Id. No. 203, Seq. Id. No. 204, Seq. Id. No. 205,Seq. Id. No. 206, Seq. Id. No. 207, Seq. Id. No. 208, Seq. Id. No. 209,Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No. 213,Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No. 217,Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id. No. 220, Seq. Id. No. 221,Seq. Id. No. 222, Seq. Id. No. 223, Seq. Id. No. 224, Seq. Id. No. 225,Seq. Id. No. 226, Seq. Id. No. 227, Seq. Id. No. 228, Seq. Id. No. 229,Seq. Id. No. 230, Seq. Id. No. 231, Seq. Id. No. 232, Seq. Id. No. 233,Seq. Id. No. 234, Seq. Id. No. 235, Seq. Id. No. 236, Seq. Id. No. 237,Seq. Id. No. 238, Seq. Id. No. 239, Seq. Id. No. 240, Seq. Id. No. 241,Seq. Id. No. 242, Seq. Id. No. 243, Seq. Id. No. 244, Seq. Id. No. 245,Seq. Id. No. 246, Seq. Id. No. 247, Seq. Id. No. 248, Seq. Id. No. 249,Seq. Id. No. 250, Seq. Id. No. 251, Seq. Id. No. 252, Seq. Id. No. 253,Seq. Id. No. 254, Seq. Id. No. 255, Seq. Id. No. 256, Seq. Id. No. 257,Seq. Id. No. 258, Seq. Id. No. 259, Seq. Id. No. 260, Seq. Id. No. 261,Seq. Id. No. 262, Seq. Id. No. 263, Seq. Id. No. 264, Seq. Id. No. 265,Seq. Id. No. 266, Seq. Id. No. 267, Seq. Id. No. 268, Seq. Id. No. 269,Seq. Id. No. 270, Seq. Id. No. 271, Seq. Id. No. 272, Seq. Id. No. 273,Seq. Id. No. 274, Seq. Id. No. 275, Seq. Id. No. 276, Seq. Id. No. 277,Seq. Id. No. 278, Seq. Id. No. 279, Seq. Id. No. 280, Seq. Id. No. 281,Seq. Id. No. 282, Seq. Id. No. 283, Seq. Id. No. 284, Seq. Id. No. 285,Seq. Id. No. 286, Seq. Id. No. 287, Seq. Id. No. 288, Seq. Id. No. 289,Seq. Id. No. 290, Seq. Id. No. 291, Seq. Id. No. 292, Seq. Id. No. 293,Seq. Id. No. 294, Seq. Id. No. 295, Seq. Id. No. 296, Seq. Id. No. 297,Seq. Id. No. 298, Seq. Id. No. 299, Seq. Id. No. 300, Seq. Id. No. 301,Seq. Id. No. 302, Seq. Id. No. 303, Seq. Id. No. 304, Seq. Id. No. 305,Seq. Id. No. 306, Seq. Id. No. 307, Seq. Id. No. 308, Seq. Id. No. 310,Seq. Id. No. 311, Seq. Id. No. 312, Seq. Id. No. 313, Seq. Id. No. 314,Seq. Id. No. 315, Seq. Id. No. 316, Seq. Id. No. 317, Seq. Id. No. 318,Seq. Id. No. 319, Seq. Id. No. 320, Seq. Id. No. 321, Seq. Id. No. 322,Seq. Id. No. 323, Seq. Id. No. 324, Seq. Id. No. 325, Seq. Id. No. 326,Seq. Id. No. 327, Seq. Id. No. 328, Seq. Id. No. 329, Seq. Id. No. 330,Seq. Id. No. 331, Seq. Id. No. 332, Seq. Id. No. 333, Seq. Id. No. 334,Seq. Id. No. 335, Seq. Id. No. 336, Seq. Id. No. 337, Seq. Id. No. 338,Seq. Id. No. 339, Seq. Id. No. 340, Seq. Id. No. 341, Seq. Id. No. 342,Seq. Id. No. 343, Seq. Id. No. 344, Seq. Id. No. 345, Seq. Id. No. 346,Seq. Id. No. 347, Seq. Id. No. 348, Seq. Id. No. 349, Seq. Id. No. 350,Seq. Id. No. 351, Seq. Id. No. 352, Seq. Id. No. 353, Seq. Id. No. 354,Seq. Id. No. 355, Seq. Id. No. 356, Seq. Id. No. 357, Seq. Id. No. 358,Seq. Id. No. 359, Seq. Id. No. 360, Seq. Id. No. 361, Seq. Id. No. 362,Seq. Id. No. 363, Seq. Id. No. 364, Seq. Id. No. 365, Seq. Id. No. 366,Seq. Id. No. 367, Seq. Id. No. 368, Seq. Id. No. 369, Seq. Id. No. 371,Seq. Id. No. 372, Seq. Id. No. 374, Seq. Id. No. 375, Seq. Id. No. 376,Seq. Id. No. 377, Seq. Id. No. 378, Seq. Id. No. 379, Seq. Id. No. 380,Seq. Id. No. 381, Seq. Id. No. 382, Seq. Id. No. 383, Seq. Id. No. 384,Seq. Id. No. 385, Seq. Id. No. 386, Seq. Id. No. 387, Seq. Id. No. 388,Seq. Id. No. 389, Seq. Id. No. 390, Seq. Id. No. 391, Seq. Id. No. 392,Seq. Id. No. 393, Seq. Id. No. 394, Seq. Id. No. 395, Seq. Id. No. 396,Seq. Id. No. 397, Seq. Id. No. 398, Seq. Id. No. 399, Seq. Id. No. 400,Seq. Id. No. 401, Seq. Id. No. 402, Seq. Id. No. 403, Seq. Id. No. 404,Seq. Id. No. 405, Seq. Id. No. 406, Seq. Id. No. 407, Seq. Id. No. 408,Seq. Id. No. 409, Seq. Id. No. 410, Seq. Id. No. 411, Seq. Id. No. 412,Seq. Id. No. 413, Seq. Id. No. 414, Seq. Id. No. 415, Seq. Id. No. 416,Seq. Id. No. 417, Seq. Id. No. 418, Seq. Id. No. 419, Seq. Id. No. 420,Seq. Id. No. 421, Seq. Id. No. 422, Seq. Id. No. 423, Seq. Id. No. 424,Seq. Id. No. 425, Seq. Id. No. 426, Seq. Id. No. 427, Seq. Id. No. 428,Seq. Id. No. 429, Seq. Id. No. 430, Seq. Id. No. 431, Seq. Id. No. 432,Seq. Id. No. 433, Seq. Id. No. 434, Seq. Id. No. 435, Seq. Id. No. 436,Seq. Id. No. 437, Seq. Id. No. 438, Seq. Id. No. 439, Seq. Id. No. 440,Seq. Id. No. 441, Seq. Id. No. 442, Seq. Id. No. 443, Seq. Id. No. 444,Seq. Id. No. 445, Seq. Id. No. 446, Seq. Id. No. 447, Seq. Id. No. 448,Seq. Id. No. 449, Seq. Id. No. 450, Seq. Id. No. 451, Seq. Id. No. 452,Seq. Id. No. 453, Seq. Id. No. 454, Seq. Id. No. 455, Seq. Id. No. 456,Seq. Id. No. 457, Seq. Id. No. 458, Seq. Id. No. 459, Seq. Id. No. 460,Seq. Id. No. 461, Seq. Id. No. 462, Seq. Id. No. 463, Seq. Id. No. 464,Seq. Id. No. 465, Seq. Id. No. 466, Seq. Id. No. 467, Seq. Id. No. 468,Seq. Id. No. 469, Seq. Id. No. 470, Seq. Id. No. 471, Seq. Id. No. 472,Seq. Id. No. 473, Seq. Id. No. 474, Seq. Id. No. 475, Seq. Id. No. 476,Seq. Id. No. 477, Seq. Id. No. 478, Seq. Id. No. 479, Seq. Id. No. 480,Seq. Id. No. 481, Seq. Id. No. 482, Seq. Id. No. 483, Seq. Id. No. 484,Seq. Id. No. 485, Seq. Id. No. 486, Seq. Id. No. 487, Seq. Id. No. 488,Seq. Id. No. 489, Seq. Id. No. 490, Seq. Id. No. 491, Seq. Id. No. 492,Seq. Id. No. 493, Seq. Id. No. 494, Seq. Id. No. 495, Seq. Id. No. 496,Seq. Id. No. 497, Seq. Id. No. 498, Seq. Id. No. 499, Seq. Id. No. 500,Seq. Id. No. 501, Seq. Id. No. 502, Seq. Id. No. 503, Seq. Id. No. 504,Seq. Id. No. 505, Seq. Id. No. 506, Seq. Id. No. 507, Seq. Id. No. 508,Seq. Id. No. 509, Seq. Id. No. 510, Seq. Id. No. 511, Seq. Id. No. 512,Seq. Id. No. 513, Seq. Id. No. 514, Seq. Id. No. 515, Seq. Id. No. 516and Seq. Id. No.
 517. 6. The method according to any of claims 1-3,wherein the autoantibody recognizes a mutated EGFR peptide that isselected from the group consisting of Seq. Id. No. 518, Seq. Id. No.522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 526, Seq. Id. No.527, Seq. Id. No. 528, Seq. Id. No. 529, Seq. Id. No. 530, Seq. Id. No.531, Seq. Id. No. 532, Seq. Id. No. 533, Seq. Id. No. 534, Seq. Id. No.535, Seq. Id. No. 536, Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No.539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No.543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No.547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No.551, Seq. Id. No. 552, Seq. Id. No. 553, Seq. Id. No. 554, Seq. Id. No.555, Seq. Id. No. 557, Seq. Id. No. 559, Seq. Id. No. 560, Seq. Id. No.562, Seq. Id. No. 563, Seq. Id. No. 564, Seq. Id. No. 565, Seq. Id. No.567, Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No.571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No.575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No.579, Seq. Id. No. 580, Seq. Id. No. 581, Seq. Id. No. 582, Seq. Id. No.583, Seq. Id. No. 584, Seq. Id. No. 585, Seq. Id. No. 586, Seq. Id. No.587, Seq. Id. No. 588, Seq. Id. No. 589, Seq. Id. No. 590, Seq. Id. No.591, Seq. Id. No. 592, Seq. Id. No. 593, Seq. Id. No. 594, Seq. Id. No.595, Seq. Id. No. 596, Seq. Id. No. 597, Seq. Id. No. 598, Seq. Id. No.599 and Seq. Id. No.
 601. 7. The method according to any of claims 1-3,wherein the autoantibody recognizes a mutated EGFR peptide that isselected from the group consisting of Seq. Id. No. 604, Seq. Id. No.605, Seq. Id. No. 606, Seq. Id. No. 607, Seq. Id. No. 608, Seq. Id. No.609, Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No.613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No.617, Seq. Id. No. 618 and Seq. Id. No.
 619. 8. The method according toany of claims 1-7, wherein the level of an autoantibody in the bloodsample of the human subject is 5 times higher than the level of saidautoantibody representative for a human subject of a healthy population.9. Erlotinib or a pharmaceutically acceptable salt thereof, inparticular erlotinib hydrochloride, for use in treating a NSCLC patientidentified by a method of any of claims 1 to 8 comprising administeringerlotinib or a pharmaceutically acceptable salt thereof, in particularerlotinib hydrochloride to the patient.
 10. Use of an autoantibody forpredicting the response of a NSCLC patient to erlotinib or apharmaceutically acceptable salt thereof, in particular erlotinibhydrochloride, treatment, which antibody was identified by a method ofany of claims 1 to
 8. 11. A kit for detecting in a blood sample of thehuman subject a level of one or more autoantibodies selected from thegroup of autoantibodies recognizing mutated human EGFR, wherein anincreased level of said autoantibodies selected from the group ofautoantibodies recognizing mutated human EGFR in the blood sample of thehuman subject compared to a level of said autoantibodies representativefor a human subject of a healthy population is indicative for non-smallcell lung cancer.
 12. A kit according to claim 11, wherein theautoantibody recognizes a mutated EGFR peptide that is selected from thegroup consisting of Seq. Id. No. 554, Seq. Id. No. 555, Seq. Id. No.556, Seq. Id. No. 557, Seq. Id. No. 558, Seq. Id. No. 559, and Seq. Id.No.
 560. 13. A kit according to claim 11, wherein the autoantibodyrecognizes a mutated EGFR peptide that is selected from the groupconsisting of Seq. Id. No. 1, Seq. Id. No. 2, Seq. Id. No. 4, Seq. Id.No. 5, Seq. Id. No. 6, Seq. Id. No. 7 and Seq. Id. No.
 15. 14. A kitaccording to claim 11, wherein the autoantibody recognizes a mutatedEGFR peptide that is selected from the group consisting of Seq. Id. No.16, Seq. Id. No. 17, Seq. Id. No. 18, Seq. Id. No. 19, Seq. Id. No. 20,Seq. Id. No. 21, Seq. Id. No. 22, Seq. Id. No. 23, Seq. Id. No. 24, Seq.Id. No. 25, Seq. Id. No. 26, Seq. Id. No. 27, Seq. Id. No. 28, Seq. Id.No. 29, Seq. Id. No. 30, Seq. Id. No. 31, Seq. Id. No. 32, Seq. Id. No.33, Seq. Id. No. 34, Seq. Id. No. 35, Seq. Id. No. 36, Seq. Id. No. 37,Seq. Id. No. 38, Seq. Id. No. 39, Seq. Id. No. 40, Seq. Id. No. 41, Seq.Id. No. 42, Seq. Id. No. 43, Seq. Id. No. 44, Seq. Id. No. 45, Seq. Id.No. 46, Seq. Id. No. 47, Seq. Id. No. 48, Seq. Id. No. 49, Seq. Id. No.50, Seq. Id. No. 51, Seq. Id. No. 52, Seq. Id. No. 53, Seq. Id. No. 54,Seq. Id. No. 55, Seq. Id. No. 56, Seq. Id. No. 57, Seq. Id. No. 58, Seq.Id. No. 59, Seq. Id. No. 60, Seq. Id. No. 61, Seq. Id. No. 62, Seq. Id.No. 63, Seq. Id. No. 65, Seq. Id. No. 66, Seq. Id. No. 67, Seq. Id. No.68, Seq. Id. No. 69, Seq. Id. No. 70, Seq. Id. No. 71, Seq. Id. No. 72,Seq. Id. No. 73, Seq. Id. No. 74, Seq. Id. No. 75, Seq. Id. No. 76, Seq.Id. No. 77, Seq. Id. No. 78, Seq. Id. No. 79, Seq. Id. No. 80, Seq. Id.No. 81, Seq. Id. No. 82, Seq. Id. No. 83, Seq. Id. No. 84, Seq. Id. No.85, Seq. Id. No. 86, Seq. Id. No. 87, Seq. Id. No. 88, Seq. Id. No. 89,Seq. Id. No. 90, Seq. Id. No. 91, Seq. Id. No. 92, Seq. Id. No. 93, Seq.Id. No. 94, Seq. Id. No. 95, Seq. Id. No. 96, Seq. Id. No. 97, Seq. Id.No. 98, Seq. Id. No. 99, Seq. Id. No. 100, Seq. Id. No. 101, Seq. Id.No. 103, Seq. Id. No. 104, Seq. Id. No. 105, Seq. Id. No. 106, Seq. Id.No. 107, Seq. Id. No. 108, Seq. Id. No. 109, Seq. Id. No. 110, Seq. Id.No. 111, Seq. Id. No. 112, Seq. Id. No. 113, Seq. Id. No. 114, Seq. Id.No. 115, Seq. Id. No. 116, Seq. Id. No. 117, Seq. Id. No. 118, Seq. Id.No. 119, Seq. Id. No. 120, Seq. Id. No. 121, Seq. Id. No. 122, Seq. Id.No. 123, Seq. Id. No. 124, Seq. Id. No. 125, Seq. Id. No. 126, Seq. Id.No. 127, Seq. Id. No. 128, Seq. Id. No. 129, Seq. Id. No. 130, Seq. Id.No. 131, Seq. Id. No. 132, Seq. Id. No. 133, Seq. Id. No. 134, Seq. Id.No. 135, Seq. Id. No. 136, Seq. Id. No. 137, Seq. Id. No. 138, Seq. Id.No. 139, Seq. Id. No. 140, Seq. Id. No. 141, Seq. Id. No. 142, Seq. Id.No. 143, Seq. Id. No. 144, Seq. Id. No. 145, Seq. Id. No. 146, Seq. Id.No. 147, Seq. Id. No. 148, Seq. Id. No. 149, Seq. Id. No. 150, Seq. Id.No. 151, Seq. Id. No. 152, Seq. Id. No. 153, Seq. Id. No. 154, Seq. Id.No. 155, Seq. Id. No. 156, Seq. Id. No. 157, Seq. Id. No. 158, Seq. Id.No. 159, Seq. Id. No. 160, Seq. Id. No. 161, Seq. Id. No. 162, Seq. Id.No. 163, Seq. Id. No. 164, Seq. Id. No. 165, Seq. Id. No. 166, Seq. Id.No. 167, Seq. Id. No. 168, Seq. Id. No. 169, Seq. Id. No. 170, Seq. Id.No. 171, Seq. Id. No. 172, Seq. Id. No. 173, Seq. Id. No. 174, Seq. Id.No. 175, Seq. Id. No. 176, Seq. Id. No. 177, Seq. Id. No. 178, Seq. Id.No. 179, Seq. Id. No. 180, Seq. Id. No. 181, Seq. Id. No. 182, Seq. Id.No. 183, Seq. Id. No. 184, Seq. Id. No. 185, Seq. Id. No. 186, Seq. Id.No. 187, Seq. Id. No. 188, Seq. Id. No. 189, Seq. Id. No. 190, Seq. Id.No. 191, Seq. Id. No. 192, Seq. Id. No. 193, Seq. Id. No. 194, Seq. Id.No. 195, Seq. Id. No. 196, Seq. Id. No. 197, Seq. Id. No. 198, Seq. Id.No. 199, Seq. Id. No. 200, Seq. Id. No. 201, Seq. Id. No. 202, Seq. Id.No. 203, Seq. Id. No. 204, Seq. Id. No. 205, Seq. Id. No. 206, Seq. Id.No. 207, Seq. Id. No. 208, Seq. Id. No. 209, Seq. Id. No. 210, Seq. Id.No. 211, Seq. Id. No. 212, Seq. Id. No. 213, Seq. Id. No. 214, Seq. Id.No. 215, Seq. Id. No. 216, Seq. Id. No. 217, Seq. Id. No. 218, Seq. Id.No. 219, Seq. Id. No. 220, Seq. Id. No. 221, Seq. Id. No. 222, Seq. Id.No. 223, Seq. Id. No. 224, Seq. Id. No. 225, Seq. Id. No. 226, Seq. Id.No. 227, Seq. Id. No. 228, Seq. Id. No. 229, Seq. Id. No. 230, Seq. Id.No. 231, Seq. Id. No. 232, Seq. Id. No. 233, Seq. Id. No. 234, Seq. Id.No. 235, Seq. Id. No. 236, Seq. Id. No. 237, Seq. Id. No. 238, Seq. Id.No. 239, Seq. Id. No. 240, Seq. Id. No. 241, Seq. Id. No. 242, Seq. Id.No. 243, Seq. Id. No. 244, Seq. Id. No. 245, Seq. Id. No. 246, Seq. Id.No. 247, Seq. Id. No. 248, Seq. Id. No. 249, Seq. Id. No. 250, Seq. Id.No. 251, Seq. Id. No. 252, Seq. Id. No. 253, Seq. Id. No. 254, Seq. Id.No. 255, Seq. Id. No. 256, Seq. Id. No. 257, Seq. Id. No. 258, Seq. Id.No. 259, Seq. Id. No. 260, Seq. Id. No. 261, Seq. Id. No. 262, Seq. Id.No. 263, Seq. Id. No. 264, Seq. Id. No. 265, Seq. Id. No. 266, Seq. Id.No. 267, Seq. Id. No. 268, Seq. Id. No. 269, Seq. Id. No. 270, Seq. Id.No. 271, Seq. Id. No. 272, Seq. Id. No. 273, Seq. Id. No. 274, Seq. Id.No. 275, Seq. Id. No. 276, Seq. Id. No. 277, Seq. Id. No. 278, Seq. Id.No. 279, Seq. Id. No. 280, Seq. Id. No. 281, Seq. Id. No. 282, Seq. Id.No. 283, Seq. Id. No. 284, Seq. Id. No. 285, Seq. Id. No. 286, Seq. Id.No. 287, Seq. Id. No. 288, Seq. Id. No. 289, Seq. Id. No. 290, Seq. Id.No. 291, Seq. Id. No. 292, Seq. Id. No. 293, Seq. Id. No. 294, Seq. Id.No. 295, Seq. Id. No. 296, Seq. Id. No. 297, Seq. Id. No. 298, Seq. Id.No. 299, Seq. Id. No. 300, Seq. Id. No. 301, Seq. Id. No. 302, Seq. Id.No. 303, Seq. Id. No. 304, Seq. Id. No. 305, Seq. Id. No. 306, Seq. Id.No. 307, Seq. Id. No. 308, Seq. Id. No. 310, Seq. Id. No. 311, Seq. Id.No. 312, Seq. Id. No. 313, Seq. Id. No. 314, Seq. Id. No. 315, Seq. Id.No. 316, Seq. Id. No. 317, Seq. Id. No. 318, Seq. Id. No. 319, Seq. Id.No. 320, Seq. Id. No. 321, Seq. Id. No. 322, Seq. Id. No. 323, Seq. Id.No. 324, Seq. Id. No. 325, Seq. Id. No. 326, Seq. Id. No. 327, Seq. Id.No. 328, Seq. Id. No. 329, Seq. Id. No. 330, Seq. Id. No. 331, Seq. Id.No. 332, Seq. Id. No. 333, Seq. Id. No. 334, Seq. Id. No. 335, Seq. Id.No. 336, Seq. Id. No. 337, Seq. Id. No. 338, Seq. Id. No. 339, Seq. Id.No. 340, Seq. Id. No. 341, Seq. Id. No. 342, Seq. Id. No. 343, Seq. Id.No. 344, Seq. Id. No. 345, Seq. Id. No. 346, Seq. Id. No. 347, Seq. Id.No. 348, Seq. Id. No. 349, Seq. Id. No. 350, Seq. Id. No. 351, Seq. Id.No. 352, Seq. Id. No. 353, Seq. Id. No. 354, Seq. Id. No. 355, Seq. Id.No. 356, Seq. Id. No. 357, Seq. Id. No. 358, Seq. Id. No. 359, Seq. Id.No. 360, Seq. Id. No. 361, Seq. Id. No. 362, Seq. Id. No. 363, Seq. Id.No. 364, Seq. Id. No. 365, Seq. Id. No. 366, Seq. Id. No. 367, Seq. Id.No. 368, Seq. Id. No. 369, Seq. Id. No. 371, Seq. Id. No. 372, Seq. Id.No. 374, Seq. Id. No. 375, Seq. Id. No. 376, Seq. Id. No. 377, Seq. Id.No. 378, Seq. Id. No. 379, Seq. Id. No. 380, Seq. Id. No. 381, Seq. Id.No. 382, Seq. Id. No. 383, Seq. Id. No. 384, Seq. Id. No. 385, Seq. Id.No. 386, Seq. Id. No. 387, Seq. Id. No. 388, Seq. Id. No. 389, Seq. Id.No. 390, Seq. Id. No. 391, Seq. Id. No. 392, Seq. Id. No. 393, Seq. Id.No. 394, Seq. Id. No. 395, Seq. Id. No. 396, Seq. Id. No. 397, Seq. Id.No. 398, Seq. Id. No. 399, Seq. Id. No. 400, Seq. Id. No. 401, Seq. Id.No. 402, Seq. Id. No. 403, Seq. Id. No. 404, Seq. Id. No. 405, Seq. Id.No. 406, Seq. Id. No. 407, Seq. Id. No. 408, Seq. Id. No. 409, Seq. Id.No. 410, Seq. Id. No. 411, Seq. Id. No. 412, Seq. Id. No. 413, Seq. Id.No. 414, Seq. Id. No. 415, Seq. Id. No. 416, Seq. Id. No. 417, Seq. Id.No. 418, Seq. Id. No. 419, Seq. Id. No. 420, Seq. Id. No. 421, Seq. Id.No. 422, Seq. Id. No. 423, Seq. Id. No. 424, Seq. Id. No. 425, Seq. Id.No. 426, Seq. Id. No. 427, Seq. Id. No. 428, Seq. Id. No. 429, Seq. Id.No. 430, Seq. Id. No. 431, Seq. Id. No. 432, Seq. Id. No. 433, Seq. Id.No. 434, Seq. Id. No. 435, Seq. Id. No. 436, Seq. Id. No. 437, Seq. Id.No. 438, Seq. Id. No. 439, Seq. Id. No. 440, Seq. Id. No. 441, Seq. Id.No. 442, Seq. Id. No. 443, Seq. Id. No. 444, Seq. Id. No. 445, Seq. Id.No. 446, Seq. Id. No. 447, Seq. Id. No. 448, Seq. Id. No. 449, Seq. Id.No. 450, Seq. Id. No. 451, Seq. Id. No. 452, Seq. Id. No. 453, Seq. Id.No. 454, Seq. Id. No. 455, Seq. Id. No. 456, Seq. Id. No. 457, Seq. Id.No. 458, Seq. Id. No. 459, Seq. Id. No. 460, Seq. Id. No. 461, Seq. Id.No. 462, Seq. Id. No. 463, Seq. Id. No. 464, Seq. Id. No. 465, Seq. Id.No. 466, Seq. Id. No. 467, Seq. Id. No. 468, Seq. Id. No. 469, Seq. Id.No. 470, Seq. Id. No. 471, Seq. Id. No. 472, Seq. Id. No. 473, Seq. Id.No. 474, Seq. Id. No. 475, Seq. Id. No. 476, Seq. Id. No. 477, Seq. Id.No. 478, Seq. Id. No. 479, Seq. Id. No. 480, Seq. Id. No. 481, Seq. Id.No. 482, Seq. Id. No. 483, Seq. Id. No. 484, Seq. Id. No. 485, Seq. Id.No. 486, Seq. Id. No. 487, Seq. Id. No. 488, Seq. Id. No. 489, Seq. Id.No. 490, Seq. Id. No. 491, Seq. Id. No. 492, Seq. Id. No. 493, Seq. Id.No. 494, Seq. Id. No. 495, Seq. Id. No. 496, Seq. Id. No. 497, Seq. Id.No. 498, Seq. Id. No. 499, Seq. Id. No. 500, Seq. Id. No. 501, Seq. Id.No. 502, Seq. Id. No. 503, Seq. Id. No. 504, Seq. Id. No. 505, Seq. Id.No. 506, Seq. Id. No. 507, Seq. Id. No. 508, Seq. Id. No. 509, Seq. Id.No. 510, Seq. Id. No. 511, Seq. Id. No. 512, Seq. Id. No. 513, Seq. Id.No. 514, Seq. Id. No. 515, Seq. Id. No. 516 and Seq. Id. No.
 517. 15. Akit according to claim 11, wherein the autoantibody recognizes a mutatedEGFR peptide that is selected from the group consisting of Seq. Id. No.518, Seq. Id. No. 522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No.526, Seq. Id. No. 527, Seq. Id. No. 528, Seq. Id. No. 529, Seq. Id. No.530, Seq. Id. No. 531, Seq. Id. No. 532, Seq. Id. No. 533, Seq. Id. No.534, Seq. Id. No. 535, Seq. Id. No. 536, Seq. Id. No. 537, Seq. Id. No.538, Seq. Id. No. 539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No.542, Seq. Id. No. 543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No.546, Seq. Id. No. 547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No.550, Seq. Id. No. 551, Seq. Id. No. 552, Seq. Id. No. 553, Seq. Id. No.554, Seq. Id. No. 555, Seq. Id. No. 557, Seq. Id. No. 559, Seq. Id. No.560, Seq. Id. No. 562, Seq. Id. No. 563, Seq. Id. No. 564, Seq. Id. No.565, Seq. Id. No. 567, Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No.570, Seq. Id. No. 571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No.574, Seq. Id. No. 575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No.578, Seq. Id. No. 579, Seq. Id. No. 580, Seq. Id. No. 581, Seq. Id. No.582, Seq. Id. No. 583, Seq. Id. No. 584, Seq. Id. No. 585, Seq. Id. No.586, Seq. Id. No. 587, Seq. Id. No. 588, Seq. Id. No. 589, Seq. Id. No.590, Seq. Id. No. 591, Seq. Id. No. 592, Seq. Id. No. 593, Seq. Id. No.594, Seq. Id. No. 595, Seq. Id. No. 596, Seq. Id. No. 597, Seq. Id. No.598, Seq. Id. No. 599 and Seq. Id. No.
 601. 16. A kit according to claim11, wherein the autoantibody recognizes a mutated EGFR peptide that isselected from the group consisting of Seq. Id. No. 604, Seq. Id. No.605, Seq. Id. No. 606, Seq. Id. No. 607, Seq. Id. No. 608, Seq. Id. No.609, Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No.613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No.617, Seq. Id. No. 618 and Seq. Id. No.
 619. 17. A method of diagnosis ofnon-small cell lung cancer in a human subject comprising: measuring in ablood sample of the human subject a level of an autoantibody selectedfrom the group of autoantibodies recognizing human EGFR, wherein anincreased level of said autoantibody selected from the group ofautoantibodies recognizing human EGFR in the blood sample of the humansubject compared to a level of said autoantibody representative for ahuman subject of a healthy population is indicative for non-small celllung cancer.
 18. The method according to claim 16, wherein theautoantibody recognizes a mutated EGFR peptide is selected from thegroup consisting of Seq. Id. No. 519, Seq. Id. No. 520, Seq. Id. No. 521and Seq. Id. No.
 561. 19. The method according to any of claims 17-18,wherein the autoantibody recognizes an EGFR peptide is selected from thegroup consisting of Seq. Id. No. 1, Seq. Id. No. 2, Seq. Id. No. 3, Seq.Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7, Seq. Id. No.8, Seq. Id. No. 9, Seq. Id. No. 10, Seq. Id. No. 11, Seq. Id. No. 12,Seq. Id. No. 13, Seq. Id. No. 14, Seq. Id. No. 15, Seq. Id. No. 16, Seq.Id. No. 17, Seq. Id. No. 18, Seq. Id. No. 19, Seq. Id. No. 20, Seq. Id.No. 21, Seq. Id. No. 22, Seq. Id. No. 23, Seq. Id. No. 24, Seq. Id. No.25, Seq. Id. No. 26, Seq. Id. No. 27, Seq. Id. No. 28, Seq. Id. No. 29,Seq. Id. No. 30, Seq. Id. No. 31, Seq. Id. No. 32, Seq. Id. No. 33, Seq.Id. No. 34, Seq. Id. No. 35, Seq. Id. No. 36, Seq. Id. No. 37, Seq. Id.No. 38, Seq. Id. No. 39, Seq. Id. No. 40, Seq. Id. No. 41, Seq. Id. No.42, Seq. Id. No. 43, Seq. Id. No. 44, Seq. Id. No. 45, Seq. Id. No. 46,Seq. Id. No. 47, Seq. Id. No. 48, Seq. Id. No. 49, Seq. Id. No. 50, Seq.Id. No. 51, Seq. Id. No. 52, Seq. Id. No. 53, Seq. Id. No. 54, Seq. Id.No. 55, Seq. Id. No. 56, Seq. Id. No. 57, Seq. Id. No. 58, Seq. Id. No.59, Seq. Id. No. 60, Seq. Id. No. 61, Seq. Id. No. 62, Seq. Id. No. 63,Seq. Id. No. 64, Seq. Id. No. 65, Seq. Id. No. 66, Seq. Id. No. 67, Seq.Id. No. 68, Seq. Id. No. 69, Seq. Id. No. 70, Seq. Id. No. 71, Seq. Id.No. 72, Seq. Id. No. 73, Seq. Id. No. 74, Seq. Id. No. 75, Seq. Id. No.76, Seq. Id. No. 77, Seq. Id. No. 78, Seq. Id. No. 79, Seq. Id. No. 80,Seq. Id. No. 81, Seq. Id. No. 82, Seq. Id. No. 83, Seq. Id. No. 84, Seq.Id. No. 85, Seq. Id. No. 86, Seq. Id. No. 87, Seq. Id. No. 88, Seq. Id.No. 89, Seq. Id. No. 90, Seq. Id. No. 91, Seq. Id. No. 92, Seq. Id. No.93, Seq. Id. No. 94, Seq. Id. No. 95, Seq. Id. No. 96, Seq. Id. No. 97,Seq. Id. No. 98, Seq. Id. No. 99, Seq. Id. No. 100, Seq. Id. No. 101,Seq. Id. No. 102, Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No. 105,Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No. 109,Seq. Id. No. 110, Seq. Id. No. 111, Seq. Id. No. 112, Seq. Id. No. 113,Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No. 117,Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No. 121,Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No. 125,Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No. 129,Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No. 133,Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No. 137,Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No. 141,Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No. 145,Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No. 149,Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No. 153,Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No. 157,Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No. 161,Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No. 165,Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No. 169,Seq. Id. No. 170, Seq. Id. No. 171, Seq. Id. No. 172, Seq. Id. No. 173,Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No. 177,Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No. 181,Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No. 185,Seq. Id. No. 186, Seq. Id. No. 187, Seq. Id. No. 188, Seq. Id. No. 189,Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No. 193,Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No. 197,Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id. No. 200, Seq. Id. No. 201,Seq. Id. No. 202, Seq. Id. No. 203, Seq. Id. No. 204, Seq. Id. No. 205,Seq. Id. No. 206, Seq. Id. No. 207, Seq. Id. No. 208, Seq. Id. No. 209,Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No. 213,Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No. 217,Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id. No. 220, Seq. Id. No. 221,Seq. Id. No. 222, Seq. Id. No. 223, Seq. Id. No. 224, Seq. Id. No. 225,Seq. Id. No. 226, Seq. Id. No. 227, Seq. Id. No. 228, Seq. Id. No. 229,Seq. Id. No. 230, Seq. Id. No. 231, Seq. Id. No. 232, Seq. Id. No. 233,Seq. Id. No. 234, Seq. Id. No. 235, Seq. Id. No. 236, Seq. Id. No. 237,Seq. Id. No. 238, Seq. Id. No. 239, Seq. Id. No. 240, Seq. Id. No. 241,Seq. Id. No. 242, Seq. Id. No. 243, Seq. Id. No. 244, Seq. Id. No. 245,Seq. Id. No. 246, Seq. Id. No. 247, Seq. Id. No. 248, Seq. Id. No. 249,Seq. Id. No. 250, Seq. Id. No. 251, Seq. Id. No. 252, Seq. Id. No. 253,Seq. Id. No. 254, Seq. Id. No. 255, Seq. Id. No. 256, Seq. Id. No. 257,Seq. Id. No. 258, Seq. Id. No. 259, Seq. Id. No. 260, Seq. Id. No. 261,Seq. Id. No. 262, Seq. Id. No. 263, Seq. Id. No. 264, Seq. Id. No. 265,Seq. Id. No. 266, Seq. Id. No. 267, Seq. Id. No. 268, Seq. Id. No. 269,Seq. Id. No. 270, Seq. Id. No. 271, Seq. Id. No. 272, Seq. Id. No. 273,Seq. Id. No. 274, Seq. Id. No. 275, Seq. Id. No. 276, Seq. Id. No. 277,Seq. Id. No. 278, Seq. Id. No. 279, Seq. Id. No. 280, Seq. Id. No. 281,Seq. Id. No. 282, Seq. Id. No. 283, Seq. Id. No. 284, Seq. Id. No. 285,Seq. Id. No. 286, Seq. Id. No. 287, Seq. Id. No. 288, Seq. Id. No. 289,Seq. Id. No. 290, Seq. Id. No. 291, Seq. Id. No. 292, Seq. Id. No. 293,Seq. Id. No. 294, Seq. Id. No. 295, Seq. Id. No. 296, Seq. Id. No. 297,Seq. Id. No. 298, Seq. Id. No. 299, Seq. Id. No. 300, Seq. Id. No. 301,Seq. Id. No. 302, Seq. Id. No. 303, Seq. Id. No. 304, Seq. Id. No. 305,Seq. Id. No. 306, Seq. Id. No. 307, Seq. Id. No. 308, Seq. Id. No. 309,Seq. Id. No. 310, Seq. Id. No. 311, Seq. Id. No. 312, Seq. Id. No. 313,Seq. Id. No. 314, Seq. Id. No. 315, Seq. Id. No. 316, Seq. Id. No. 317,Seq. Id. No. 318, Seq. Id. No. 319, Seq. Id. No. 320, Seq. Id. No. 321,Seq. Id. No. 322, Seq. Id. No. 323, Seq. Id. No. 324, Seq. Id. No. 325,Seq. Id. No. 326, Seq. Id. No. 327, Seq. Id. No. 328, Seq. Id. No. 329,Seq. Id. No. 330, Seq. Id. No. 331, Seq. Id. No. 332, Seq. Id. No. 333,Seq. Id. No. 334, Seq. Id. No. 335, Seq. Id. No. 336, Seq. Id. No. 337,Seq. Id. No. 338, Seq. Id. No. 339, Seq. Id. No. 340, Seq. Id. No. 341,Seq. Id. No. 342, Seq. Id. No. 343, Seq. Id. No. 344, Seq. Id. No. 345,Seq. Id. No. 346, Seq. Id. No. 347, Seq. Id. No. 348, Seq. Id. No. 349,Seq. Id. No. 350, Seq. Id. No. 351, Seq. Id. No. 352, Seq. Id. No. 353,Seq. Id. No. 354, Seq. Id. No. 355, Seq. Id. No. 356, Seq. Id. No. 357,Seq. Id. No. 358, Seq. Id. No. 359, Seq. Id. No. 360, Seq. Id. No. 361,Seq. Id. No. 362, Seq. Id. No. 363, Seq. Id. No. 364, Seq. Id. No. 365,Seq. Id. No. 366, Seq. Id. No. 367, Seq. Id. No. 368, Seq. Id. No. 369,Seq. Id. No. 370, Seq. Id. No. 371, Seq. Id. No. 372, Seq. Id. No. 373,Seq. Id. No. 374, Seq. Id. No. 375, Seq. Id. No. 376, Seq. Id. No. 377,Seq. Id. No. 378, Seq. Id. No. 379, Seq. Id. No. 380, Seq. Id. No. 381,Seq. Id. No. 382, Seq. Id. No. 383, Seq. Id. No. 384, Seq. Id. No. 385,Seq. Id. No. 386, Seq. Id. No. 387, Seq. Id. No. 388, Seq. Id. No. 389,Seq. Id. No. 390, Seq. Id. No. 391, Seq. Id. No. 392, Seq. Id. No. 393,Seq. Id. No. 394, Seq. Id. No. 395, Seq. Id. No. 396, Seq. Id. No. 397,Seq. Id. No. 398, Seq. Id. No. 399, Seq. Id. No. 400, Seq. Id. No. 401,Seq. Id. No. 402, Seq. Id. No. 403, Seq. Id. No. 404, Seq. Id. No. 405,Seq. Id. No. 406, Seq. Id. No. 407, Seq. Id. No. 408, Seq. Id. No. 409,Seq. Id. No. 410, Seq. Id. No. 411, Seq. Id. No. 412, Seq. Id. No. 413,Seq. Id. No. 414, Seq. Id. No. 415, Seq. Id. No. 416, Seq. Id. No. 417,Seq. Id. No. 418, Seq. Id. No. 419, Seq. Id. No. 420, Seq. Id. No. 421,Seq. Id. No. 422, Seq. Id. No. 423, Seq. Id. No. 424, Seq. Id. No. 425,Seq. Id. No. 426, Seq. Id. No. 427, Seq. Id. No. 428, Seq. Id. No. 429,Seq. Id. No. 430, Seq. Id. No. 431, Seq. Id. No. 432, Seq. Id. No. 433,Seq. Id. No. 434, Seq. Id. No. 435, Seq. Id. No. 436, Seq. Id. No. 437,Seq. Id. No. 438, Seq. Id. No. 439, Seq. Id. No. 440, Seq. Id. No. 441,Seq. Id. No. 442, Seq. Id. No. 443, Seq. Id. No. 444, Seq. Id. No. 445,Seq. Id. No. 446, Seq. Id. No. 447, Seq. Id. No. 448, Seq. Id. No. 449,Seq. Id. No. 450, Seq. Id. No. 451, Seq. Id. No. 452, Seq. Id. No. 453,Seq. Id. No. 454, Seq. Id. No. 455, Seq. Id. No. 456, Seq. Id. No. 457,Seq. Id. No. 458, Seq. Id. No. 459, Seq. Id. No. 460, Seq. Id. No. 461,Seq. Id. No. 462, Seq. Id. No. 463, Seq. Id. No. 464, Seq. Id. No. 465,Seq. Id. No. 466, Seq. Id. No. 467, Seq. Id. No. 468, Seq. Id. No. 469,Seq. Id. No. 470, Seq. Id. No. 471, Seq. Id. No. 472, Seq. Id. No. 473,Seq. Id. No. 474, Seq. Id. No. 475, Seq. Id. No. 476, Seq. Id. No. 477,Seq. Id. No. 478, Seq. Id. No. 479, Seq. Id. No. 480, Seq. Id. No. 481,Seq. Id. No. 482, Seq. Id. No. 483, Seq. Id. No. 484, Seq. Id. No. 485,Seq. Id. No. 486, Seq. Id. No. 487, Seq. Id. No. 488, Seq. Id. No. 489,Seq. Id. No. 490, Seq. Id. No. 491, Seq. Id. No. 492, Seq. Id. No. 493,Seq. Id. No. 494, Seq. Id. No. 495, Seq. Id. No. 496, Seq. Id. No. 497,Seq. Id. No. 498, Seq. Id. No. 499, Seq. Id. No. 500, Seq. Id. No. 501,Seq. Id. No. 502, Seq. Id. No. 503, Seq. Id. No. 504, Seq. Id. No. 505,Seq. Id. No. 506, Seq. Id. No. 507, Seq. Id. No. 508, Seq. Id. No. 509,Seq. Id. No. 510, Seq. Id. No. 511, Seq. Id. No. 512, Seq. Id. No. 513,Seq. Id. No. 514, Seq. Id. No. 515, Seq. Id. No. 516, Seq. Id. No. 517,Seq. Id. No. 518, Seq. Id. No. 519, Seq. Id. No. 520, Seq. Id. No. 521,Seq. Id. No. 522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 525,Seq. Id. No. 526, Seq. Id. No. 527, Seq. Id. No. 528, Seq. Id. No. 529,Seq. Id. No. 530, Seq. Id. No. 531, Seq. Id. No. 532, Seq. Id. No. 533,Seq. Id. No. 534, Seq. Id. No. 535, Seq. Id. No. 536, Seq. Id. No. 537,Seq. Id. No. 538, Seq. Id. No. 539, Seq. Id. No. 540, Seq. Id. No. 541,Seq. Id. No. 542, Seq. Id. No. 543, Seq. Id. No. 544, Seq. Id. No. 545,Seq. Id. No. 546, Seq. Id. No. 547, Seq. Id. No. 548, Seq. Id. No. 549,Seq. Id. No. 550, Seq. Id. No. 551, Seq. Id. No. 552, Seq. Id. No. 553,Seq. Id. No. 554, Seq. Id. No. 555, Seq. Id. No. 556, Seq. Id. No. 557,Seq. Id. No. 558, Seq. Id. No. 559, Seq. Id. No. 560, Seq. Id. No. 561,Seq. Id. No. 562, Seq. Id. No. 563, Seq. Id. No. 564, Seq. Id. No. 565,Seq. Id. No. 566, Seq. Id. No. 567, Seq. Id. No. 568, Seq. Id. No. 569,Seq. Id. No. 570, Seq. Id. No. 571, Seq. Id. No. 572, Seq. Id. No. 573,Seq. Id. No. 574, Seq. Id. No. 575, Seq. Id. No. 576, Seq. Id. No. 577,Seq. Id. No. 578, Seq. Id. No. 579, Seq. Id. No. 580, Seq. Id. No. 581,Seq. Id. No. 582, Seq. Id. No. 583, Seq. Id. No. 584, Seq. Id. No. 585,Seq. Id. No. 586, Seq. Id. No. 587, Seq. Id. No. 588, Seq. Id. No. 589,Seq. Id. No. 590, Seq. Id. No. 591, Seq. Id. No. 592, Seq. Id. No. 593,Seq. Id. No. 594, Seq. Id. No. 595, Seq. Id. No. 596, Seq. Id. No. 597,Seq. Id. No. 598, Seq. Id. No. 599, Seq. Id. No. 600, Seq. Id. No. 601,Seq. Id. No. 602, Seq. Id. No. 603, Seq. Id. No. 604, Seq. Id. No. 605,Seq. Id. No. 606, Seq. Id. No. 607, Seq. Id. No. 608, Seq. Id. No. 609,Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No. 613,Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No. 617,Seq. Id. No. 618, and Seq. Id. No.
 619. 20. The method according to anyof claims 19, wherein the autoantibody recognizes an EGFR peptide thatis selected from the group consisting of Seq. Id. No. 3, Seq. Id. No. 8,Seq. Id. No. 9, Seq. Id. No. 10, Seq. Id. No. 11, Seq. Id. No. 12, Seq.Id. No. 13, Seq. Id. No. 14, Seq. Id. No. 64, Seq. Id. No. 102, Seq. Id.No. 309, Seq. Id. No. 370, Seq. Id. No. 373, Seq. Id. No. 519, Seq. Id.No. 520, Seq. Id. No. 521, Seq. Id. No. 525, Seq. Id. No. 556, Seq. Id.No. 558, Seq. Id. No. 561, Seq. Id. No. 566, Seq. Id. No. 600, Seq. Id.No. 602 and Seq. Id. No.
 603. 21. The method according to any of claims17-20, wherein the level of an autoantibody in the blood sample of thehuman subject is 5 times higher than the level of said autoantibodyrepresentative for a human subject of a healthy population. 22.Erlotinib or a pharmaceutically acceptable salt thereof, in particularerlotinib hydrochloride, for use in treating a NSCLC patient identifiedby a method of any of claims 17 to 21 comprising administering erlotinibor a pharmaceutically acceptable salt thereof, in particular erlotinibhydrochloride to the patient.
 23. Use of an autoantibody for predictingthe response of a NSCLC patient to erlotinib or a pharmaceuticallyacceptable salt thereof, in particular erlotinib hydrochloride,treatment, which antibody was identified by a method of any of claims 17to
 21. 24. A kit for detecting in a blood sample of the human subject alevel of one or more autoantibodies selected from the group ofautoantibodies recognizing human EGFR, wherein an increased level ofsaid autoantibodies selected from the group of autoantibodiesrecognizing human EGFR in the blood sample of the human subject comparedto a level of said autoantibodies representative for a human subject ofa healthy population is indicative for non-small cell lung cancer.
 25. Akit according to claim 24, wherein the autoantibody recognizes an EGFRpeptide that is selected from the group consisting of Seq. Id. No. 3,Seq. Id. No. 8, Seq. Id. No. 9, Seq. Id. No. 10, Seq. Id. No. 11, Seq.Id. No. 12, Seq. Id. No. 13, Seq. Id. No. 14, Seq. Id. No. 64, Seq. Id.No. 102, Seq. Id. No. 309, Seq. Id. No. 370, Seq. Id. No. 373, Seq. Id.No. 519, Seq. Id. No. 520, Seq. Id. No. 521, Seq. Id. No. 525, Seq. Id.No. 556, Seq. Id. No. 558, Seq. Id. No. 561, Seq. Id. No. 566, Seq. Id.No. 600, Seq. Id. No. 602 and Seq. Id. No.
 603. 26. A kit according toclaim 25, wherein the autoantibody recognizes an EGFR peptide that isselected from the group consisting of Seq. Id. No. 519, Seq. Id. No.520, Seq. Id. No. 521, and Seq. Id. No.
 561. 27. A method of diagnosisof non-small cell lung cancer in a human subject comprising: a)measuring in a blood sample of the human subject a level of anautoantibody selected from the group of autoantibodies recognizingmutated human EGFR, b) comparing the level of said autoantibody to areference level, and c) providing a diagnosis of non-small cell lungcancer when the level of said autoantibody is above the reference level.28. A method of diagnosis of non-small cell lung cancer in a humansubject comprising: a) measuring in a blood sample of the human subjecta level of an autoantibody selected from the group of autoantibodiesrecognizing mutated human EGFR, b) comparing the level of saidautoantibody to a reference level, and c) recommending a treatment whenthe level of said autoantibody is above the reference level.
 29. Themethod according to claim 27 or 28, wherein the autoantibody recognizesa mutated EGFR peptide is selected from the group consisting of Seq. Id.No. 554, Seq. Id. No. 555, Seq. Id. No. 556, Seq. Id. No. 557, Seq. Id.No. 558, Seq. Id. No. 559, and Seq. Id. No.
 560. 30. The methodaccording to any of claims 28-29, wherein the autoantibody recognizes amutated EGFR peptide is selected from the group consisting of Seq. Id.No. 1, Seq. Id. No. 2, Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6,Seq. Id. No. 7, Seq. Id. No. 15, Seq. Id. No. 16, Seq. Id. No. 17, Seq.Id. No. 18, Seq. Id. No. 19, Seq. Id. No. 20, Seq. Id. No. 21, Seq. Id.No. 22, Seq. Id. No. 23, Seq. Id. No. 24, Seq. Id. No. 25, Seq. Id. No.26, Seq. Id. No. 27, Seq. Id. No. 28, Seq. Id. No. 29, Seq. Id. No. 30,Seq. Id. No. 31, Seq. Id. No. 32, Seq. Id. No. 33, Seq. Id. No. 34, Seq.Id. No. 35, Seq. Id. No. 36, Seq. Id. No. 37, Seq. Id. No. 38, Seq. Id.No. 39, Seq. Id. No. 40, Seq. Id. No. 41, Seq. Id. No. 42, Seq. Id. No.43, Seq. Id. No. 44, Seq. Id. No. 45, Seq. Id. No. 46, Seq. Id. No. 47,Seq. Id. No. 48, Seq. Id. No. 49, Seq. Id. No. 50, Seq. Id. No. 51, Seq.Id. No. 52, Seq. Id. No. 53, Seq. Id. No. 54, Seq. Id. No. 55, Seq. Id.No. 56, Seq. Id. No. 57, Seq. Id. No. 58, Seq. Id. No. 59, Seq. Id. No.60, Seq. Id. No. 61, Seq. Id. No. 62, Seq. Id. No. 63, Seq. Id. No. 65,Seq. Id. No. 66, Seq. Id. No. 67, Seq. Id. No. 68, Seq. Id. No. 69, Seq.Id. No. 70, Seq. Id. No. 71, Seq. Id. No. 72, Seq. Id. No. 73, Seq. Id.No. 74, Seq. Id. No. 75, Seq. Id. No. 76, Seq. Id. No. 77, Seq. Id. No.78, Seq. Id. No. 79, Seq. Id. No. 80, Seq. Id. No. 81, Seq. Id. No. 82,Seq. Id. No. 83, Seq. Id. No. 84, Seq. Id. No. 85, Seq. Id. No. 86, Seq.Id. No. 87, Seq. Id. No. 88, Seq. Id. No. 89, Seq. Id. No. 90, Seq. Id.No. 91, Seq. Id. No. 92, Seq. Id. No. 93, Seq. Id. No. 94, Seq. Id. No.95, Seq. Id. No. 96, Seq. Id. No. 97, Seq. Id. No. 98, Seq. Id. No. 99,Seq. Id. No. 100, Seq. Id. No. 101, Seq. Id. No. 103, Seq. Id. No. 104,Seq. Id. No. 105, Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id. No. 108,Seq. Id. No. 109, Seq. Id. No. 110, Seq. Id. No. 111, Seq. Id. No. 112,Seq. Id. No. 113, Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id. No. 116,Seq. Id. No. 117, Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id. No. 120,Seq. Id. No. 121, Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id. No. 124,Seq. Id. No. 125, Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id. No. 128,Seq. Id. No. 129, Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id. No. 132,Seq. Id. No. 133, Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id. No. 136,Seq. Id. No. 137, Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id. No. 140,Seq. Id. No. 141, Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id. No. 144,Seq. Id. No. 145, Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id. No. 148,Seq. Id. No. 149, Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id. No. 152,Seq. Id. No. 153, Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id. No. 156,Seq. Id. No. 157, Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id. No. 160,Seq. Id. No. 161, Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id. No. 164,Seq. Id. No. 165, Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id. No. 168,Seq. Id. No. 169, Seq. Id. No. 170, Seq. Id. No. 171, Seq. Id. No. 172,Seq. Id. No. 173, Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id. No. 176,Seq. Id. No. 177, Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id. No. 180,Seq. Id. No. 181, Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id. No. 184,Seq. Id. No. 185, Seq. Id. No. 186, Seq. Id. No. 187, Seq. Id. No. 188,Seq. Id. No. 189, Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id. No. 192,Seq. Id. No. 193, Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id. No. 196,Seq. Id. No. 197, Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id. No. 200,Seq. Id. No. 201, Seq. Id. No. 202, Seq. Id. No. 203, Seq. Id. No. 204,Seq. Id. No. 205, Seq. Id. No. 206, Seq. Id. No. 207, Seq. Id. No. 208,Seq. Id. No. 209, Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id. No. 212,Seq. Id. No. 213, Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id. No. 216,Seq. Id. No. 217, Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id. No. 220,Seq. Id. No. 221, Seq. Id. No. 222, Seq. Id. No. 223, Seq. Id. No. 224,Seq. Id. No. 225, Seq. Id. No. 226, Seq. Id. No. 227, Seq. Id. No. 228,Seq. Id. No. 229, Seq. Id. No. 230, Seq. Id. No. 231, Seq. Id. No. 232,Seq. Id. No. 233, Seq. Id. No. 234, Seq. Id. No. 235, Seq. Id. No. 236,Seq. Id. No. 237, Seq. Id. No. 238, Seq. Id. No. 239, Seq. Id. No. 240,Seq. Id. No. 241, Seq. Id. No. 242, Seq. Id. No. 243, Seq. Id. No. 244,Seq. Id. No. 245, Seq. Id. No. 246, Seq. Id. No. 247, Seq. Id. No. 248,Seq. Id. No. 249, Seq. Id. No. 250, Seq. Id. No. 251, Seq. Id. No. 252,Seq. Id. No. 253, Seq. Id. No. 254, Seq. Id. No. 255, Seq. Id. No. 256,Seq. Id. No. 257, Seq. Id. No. 258, Seq. Id. No. 259, Seq. Id. No. 260,Seq. Id. No. 261, Seq. Id. No. 262, Seq. Id. No. 263, Seq. Id. No. 264,Seq. Id. No. 265, Seq. Id. No. 266, Seq. Id. No. 267, Seq. Id. No. 268,Seq. Id. No. 269, Seq. Id. No. 270, Seq. Id. No. 271, Seq. Id. No. 272,Seq. Id. No. 273, Seq. Id. No. 274, Seq. Id. No. 275, Seq. Id. No. 276,Seq. Id. No. 277, Seq. Id. No. 278, Seq. Id. No. 279, Seq. Id. No. 280,Seq. Id. No. 281, Seq. Id. No. 282, Seq. Id. No. 283, Seq. Id. No. 284,Seq. Id. No. 285, Seq. Id. No. 286, Seq. Id. No. 287, Seq. Id. No. 288,Seq. Id. No. 289, Seq. Id. No. 290, Seq. Id. No. 291, Seq. Id. No. 292,Seq. Id. No. 293, Seq. Id. No. 294, Seq. Id. No. 295, Seq. Id. No. 296,Seq. Id. No. 297, Seq. Id. No. 298, Seq. Id. No. 299, Seq. Id. No. 300,Seq. Id. No. 301, Seq. Id. No. 302, Seq. Id. No. 303, Seq. Id. No. 304,Seq. Id. No. 305, Seq. Id. No. 306, Seq. Id. No. 307, Seq. Id. No. 308,Seq. Id. No. 310, Seq. Id. No. 311, Seq. Id. No. 312, Seq. Id. No. 313,Seq. Id. No. 314, Seq. Id. No. 315, Seq. Id. No. 316, Seq. Id. No. 317,Seq. Id. No. 318, Seq. Id. No. 319, Seq. Id. No. 320, Seq. Id. No. 321,Seq. Id. No. 322, Seq. Id. No. 323, Seq. Id. No. 324, Seq. Id. No. 325,Seq. Id. No. 326, Seq. Id. No. 327, Seq. Id. No. 328, Seq. Id. No. 329,Seq. Id. No. 330, Seq. Id. No. 331, Seq. Id. No. 332, Seq. Id. No. 333,Seq. Id. No. 334, Seq. Id. No. 335, Seq. Id. No. 336, Seq. Id. No. 337,Seq. Id. No. 338, Seq. Id. No. 339, Seq. Id. No. 340, Seq. Id. No. 341,Seq. Id. No. 342, Seq. Id. No. 343, Seq. Id. No. 344, Seq. Id. No. 345,Seq. Id. No. 346, Seq. Id. No. 347, Seq. Id. No. 348, Seq. Id. No. 349,Seq. Id. No. 350, Seq. Id. No. 351, Seq. Id. No. 352, Seq. Id. No. 353,Seq. Id. No. 354, Seq. Id. No. 355, Seq. Id. No. 356, Seq. Id. No. 357,Seq. Id. No. 358, Seq. Id. No. 359, Seq. Id. No. 360, Seq. Id. No. 361,Seq. Id. No. 362, Seq. Id. No. 363, Seq. Id. No. 364, Seq. Id. No. 365,Seq. Id. No. 366, Seq. Id. No. 367, Seq. Id. No. 368, Seq. Id. No. 369,Seq. Id. No. 371, Seq. Id. No. 372, Seq. Id. No. 374, Seq. Id. No. 375,Seq. Id. No. 376, Seq. Id. No. 377, Seq. Id. No. 378, Seq. Id. No. 379,Seq. Id. No. 380, Seq. Id. No. 381, Seq. Id. No. 382, Seq. Id. No. 383,Seq. Id. No. 384, Seq. Id. No. 385, Seq. Id. No. 386, Seq. Id. No. 387,Seq. Id. No. 388, Seq. Id. No. 389, Seq. Id. No. 390, Seq. Id. No. 391,Seq. Id. No. 392, Seq. Id. No. 393, Seq. Id. No. 394, Seq. Id. No. 395,Seq. Id. No. 396, Seq. Id. No. 397, Seq. Id. No. 398, Seq. Id. No. 399,Seq. Id. No. 400, Seq. Id. No. 401, Seq. Id. No. 402, Seq. Id. No. 403,Seq. Id. No. 404, Seq. Id. No. 405, Seq. Id. No. 406, Seq. Id. No. 407,Seq. Id. No. 408, Seq. Id. No. 409, Seq. Id. No. 410, Seq. Id. No. 411,Seq. Id. No. 412, Seq. Id. No. 413, Seq. Id. No. 414, Seq. Id. No. 415,Seq. Id. No. 416, Seq. Id. No. 417, Seq. Id. No. 418, Seq. Id. No. 419,Seq. Id. No. 420, Seq. Id. No. 421, Seq. Id. No. 422, Seq. Id. No. 423,Seq. Id. No. 424, Seq. Id. No. 425, Seq. Id. No. 426, Seq. Id. No. 427,Seq. Id. No. 428, Seq. Id. No. 429, Seq. Id. No. 430, Seq. Id. No. 431,Seq. Id. No. 432, Seq. Id. No. 433, Seq. Id. No. 434, Seq. Id. No. 435,Seq. Id. No. 436, Seq. Id. No. 437, Seq. Id. No. 438, Seq. Id. No. 439,Seq. Id. No. 440, Seq. Id. No. 441, Seq. Id. No. 442, Seq. Id. No. 443,Seq. Id. No. 444, Seq. Id. No. 445, Seq. Id. No. 446, Seq. Id. No. 447,Seq. Id. No. 448, Seq. Id. No. 449, Seq. Id. No. 450, Seq. Id. No. 451,Seq. Id. No. 452, Seq. Id. No. 453, Seq. Id. No. 454, Seq. Id. No. 455,Seq. Id. No. 456, Seq. Id. No. 457, Seq. Id. No. 458, Seq. Id. No. 459,Seq. Id. No. 460, Seq. Id. No. 461, Seq. Id. No. 462, Seq. Id. No. 463,Seq. Id. No. 464, Seq. Id. No. 465, Seq. Id. No. 466, Seq. Id. No. 467,Seq. Id. No. 468, Seq. Id. No. 469, Seq. Id. No. 470, Seq. Id. No. 471,Seq. Id. No. 472, Seq. Id. No. 473, Seq. Id. No. 474, Seq. Id. No. 475,Seq. Id. No. 476, Seq. Id. No. 477, Seq. Id. No. 478, Seq. Id. No. 479,Seq. Id. No. 480, Seq. Id. No. 481, Seq. Id. No. 482, Seq. Id. No. 483,Seq. Id. No. 484, Seq. Id. No. 485, Seq. Id. No. 486, Seq. Id. No. 487,Seq. Id. No. 488, Seq. Id. No. 489, Seq. Id. No. 490, Seq. Id. No. 491,Seq. Id. No. 492, Seq. Id. No. 493, Seq. Id. No. 494, Seq. Id. No. 495,Seq. Id. No. 496, Seq. Id. No. 497, Seq. Id. No. 498, Seq. Id. No. 499,Seq. Id. No. 500, Seq. Id. No. 501, Seq. Id. No. 502, Seq. Id. No. 503,Seq. Id. No. 504, Seq. Id. No. 505, Seq. Id. No. 506, Seq. Id. No. 507,Seq. Id. No. 508, Seq. Id. No. 509, Seq. Id. No. 510, Seq. Id. No. 511,Seq. Id. No. 512, Seq. Id. No. 513, Seq. Id. No. 514, Seq. Id. No. 515,Seq. Id. No. 516, Seq. Id. No. 517, Seq. Id. No. 518, Seq. Id. No. 522,Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 526, Seq. Id. No. 527,Seq. Id. No. 528, Seq. Id. No. 529, Seq. Id. No. 530, Seq. Id. No. 531,Seq. Id. No. 532, Seq. Id. No. 533, Seq. Id. No. 534, Seq. Id. No. 535,Seq. Id. No. 536, Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No. 539,Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No. 543,Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No. 547,Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No. 551,Seq. Id. No. 552, Seq. Id. No. 553, Seq. Id. No. 554, Seq. Id. No. 555,Seq. Id. No. 557, Seq. Id. No. 559, Seq. Id. No. 560, Seq. Id. No. 562,Seq. Id. No. 563, Seq. Id. No. 564, Seq. Id. No. 565, Seq. Id. No. 567,Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No. 571,Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No. 575,Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No. 579,Seq. Id. No. 580, Seq. Id. No. 581, Seq. Id. No. 582, Seq. Id. No. 583,Seq. Id. No. 584, Seq. Id. No. 585, Seq. Id. No. 586, Seq. Id. No. 587,Seq. Id. No. 588, Seq. Id. No. 589, Seq. Id. No. 590, Seq. Id. No. 591,Seq. Id. No. 592, Seq. Id. No. 593, Seq. Id. No. 594, Seq. Id. No. 595,Seq. Id. No. 596, Seq. Id. No. 597, Seq. Id. No. 598, Seq. Id. No. 599,Seq. Id. No. 601, Seq. Id. No. 604, Seq. Id. No. 605, Seq. Id. No. 606,Seq. Id. No. 607, Seq. Id. No. 608, Seq. Id. No. 609, Seq. Id. No. 610,Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No. 613, Seq. Id. No. 614,Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No. 617, Seq. Id. No. 618and Seq. Id. No.
 619. 31. The method according to any of claims 27-30,wherein the autoantibody recognizes a mutated EGFR peptide that isselected from the groups consisting of Seq. Id. No. 1, Seq. Id. No. 2,Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7 and Seq.Id. No.
 15. 32. The method according to any of claims 27-30, wherein theautoantibody recognizes a mutated EGFR peptide that is selected from thegroup consisting of Seq. Id. No. 16, Seq. Id. No. 17, Seq. Id. No. 18,Seq. Id. No. 19, Seq. Id. No. 20, Seq. Id. No. 21, Seq. Id. No. 22, Seq.Id. No. 23, Seq. Id. No. 24, Seq. Id. No. 25, Seq. Id. No. 26, Seq. Id.No. 27, Seq. Id. No. 28, Seq. Id. No. 29, Seq. Id. No. 30, Seq. Id. No.31, Seq. Id. No. 32, Seq. Id. No. 33, Seq. Id. No. 34, Seq. Id. No. 35,Seq. Id. No. 36, Seq. Id. No. 37, Seq. Id. No. 38, Seq. Id. No. 39, Seq.Id. No. 40, Seq. Id. No. 41, Seq. Id. No. 42, Seq. Id. No. 43, Seq. Id.No. 44, Seq. Id. No. 45, Seq. Id. No. 46, Seq. Id. No. 47, Seq. Id. No.48, Seq. Id. No. 49, Seq. Id. No. 50, Seq. Id. No. 51, Seq. Id. No. 52,Seq. Id. No. 53, Seq. Id. No. 54, Seq. Id. No. 55, Seq. Id. No. 56, Seq.Id. No. 57, Seq. Id. No. 58, Seq. Id. No. 59, Seq. Id. No. 60, Seq. Id.No. 61, Seq. Id. No. 62, Seq. Id. No. 63, Seq. Id. No. 65, Seq. Id. No.66, Seq. Id. No. 67, Seq. Id. No. 68, Seq. Id. No. 69, Seq. Id. No. 70,Seq. Id. No. 71, Seq. Id. No. 72, Seq. Id. No. 73, Seq. Id. No. 74, Seq.Id. No. 75, Seq. Id. No. 76, Seq. Id. No. 77, Seq. Id. No. 78, Seq. Id.No. 79, Seq. Id. No. 80, Seq. Id. No. 81, Seq. Id. No. 82, Seq. Id. No.83, Seq. Id. No. 84, Seq. Id. No. 85, Seq. Id. No. 86, Seq. Id. No. 87,Seq. Id. No. 88, Seq. Id. No. 89, Seq. Id. No. 90, Seq. Id. No. 91, Seq.Id. No. 92, Seq. Id. No. 93, Seq. Id. No. 94, Seq. Id. No. 95, Seq. Id.No. 96, Seq. Id. No. 97, Seq. Id. No. 98, Seq. Id. No. 99, Seq. Id. No.100, Seq. Id. No. 101, Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No.105, Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No.109, Seq. Id. No. 110, Seq. Id. No. 111, Seq. Id. No. 112, Seq. Id. No.113, Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No.117, Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No.121, Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No.125, Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No.129, Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No.133, Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No.137, Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No.141, Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No.145, Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No.149, Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No.153, Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No.157, Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No.161, Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No.165, Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No.169, Seq. Id. No. 170, Seq. Id. No. 171, Seq. Id. No. 172, Seq. Id. No.173, Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No.177, Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No.181, Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No.185, Seq. Id. No. 186, Seq. Id. No. 187, Seq. Id. No. 188, Seq. Id. No.189, Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No.193, Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No.197, Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id. No. 200, Seq. Id. No.201, Seq. Id. No. 202, Seq. Id. No. 203, Seq. Id. No. 204, Seq. Id. No.205, Seq. Id. No. 206, Seq. Id. No. 207, Seq. Id. No. 208, Seq. Id. No.209, Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No.213, Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No.217, Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id. No. 220, Seq. Id. No.221, Seq. Id. No. 222, Seq. Id. No. 223, Seq. Id. No. 224, Seq. Id. No.225, Seq. Id. No. 226, Seq. Id. No. 227, Seq. Id. No. 228, Seq. Id. No.229, Seq. Id. No. 230, Seq. Id. No. 231, Seq. Id. No. 232, Seq. Id. No.233, Seq. Id. No. 234, Seq. Id. No. 235, Seq. Id. No. 236, Seq. Id. No.237, Seq. Id. 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 517. 33. The method according to any of claims27-30, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the group consisting of Seq. Id. No. 518, Seq. Id. No.522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 526, Seq. Id. No.527, Seq. Id. No. 528, Seq. Id. No. 529, Seq. Id. No. 530, Seq. Id. No.531, Seq. Id. No. 532, Seq. Id. No. 533, Seq. Id. No. 534, Seq. Id. No.535, Seq. Id. No. 536, Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No.539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No.543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No.547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No.551, Seq. Id. No. 552, Seq. Id. No. 553, Seq. Id. No. 554, Seq. Id. No.555, Seq. Id. No. 557, Seq. Id. No. 559, Seq. Id. No. 560, Seq. Id. No.562, Seq. Id. No. 563, Seq. Id. No. 564, Seq. Id. No. 565, Seq. Id. No.567, Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No.571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No.575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No.579, Seq. Id. No. 580, Seq. Id. No. 581, Seq. Id. No. 582, Seq. Id. No.583, Seq. Id. No. 584, Seq. Id. No. 585, Seq. Id. No. 586, Seq. Id. No.587, Seq. Id. No. 588, Seq. Id. No. 589, Seq. Id. No. 590, Seq. Id. No.591, Seq. Id. No. 592, Seq. Id. No. 593, Seq. Id. No. 594, Seq. Id. No.595, Seq. Id. No. 596, Seq. Id. No. 597, Seq. Id. No. 598, Seq. Id. No.599 and Seq. Id. No.
 601. 34. The method according to any of claims27-30, wherein the autoantibody recognizes a mutated EGFR peptide thatis selected from the group consisting of Seq. Id. No. 604, Seq. Id. No.605, Seq. Id. No. 606, Seq. Id. No. 607, Seq. Id. No. 608, Seq. Id. No.609, Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No.613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No.617, Seq. Id. No. 618 and Seq. Id. No.
 619. 35. The method according toany of claims 27-34, wherein the level of an autoantibody in the bloodsample of the human subject is 5 times higher than the level of saidautoantibody representative for a human subject of a healthy population.36. A method of diagnosis of non-small cell lung cancer in a humansubject comprising: a) measuring in a blood sample of the human subjecta level of an autoantibody selected from the group of autoantibodiesrecognizing human EGFR, b) comparing the level of said autoantibody to areference level, and c) providing a diagnosis of non-small cell lungcancer when the level of said autoantibody is above the reference level.37. A method of diagnosis of non-small cell lung cancer in a humansubject comprising: a) measuring in a blood sample of the human subjecta level of an autoantibody selected from the group of autoantibodiesrecognizing human EGFR, b) comparing the level of said autoantibody to areference level, and c) recommending a treatment when the level of saidautoantibody is above the reference level.
 38. The method according toclaim 36 or 37, wherein the autoantibody recognizes an EGFR peptide thatis selected from the group consisting of Seq. Id. No. 3, Seq. Id. No. 8,Seq. Id. No. 9, Seq. Id. No. 10, Seq. Id. No. 11, Seq. Id. No. 12, Seq.Id. No. 13, Seq. Id. No. 14, Seq. Id. No. 64, Seq. Id. No. 102, Seq. Id.No. 309, Seq. Id. No. 370, Seq. Id. No. 373, Seq. Id. No. 519, Seq. Id.No. 520, Seq. Id. No. 521, Seq. Id. No. 525, Seq. Id. No. 556, Seq. Id.No. 558, Seq. Id. No. 561, Seq. Id. No. 566, Seq. Id. No. 600, Seq. Id.No. 602 and Seq. Id. No.
 603. 39. The method according to any of claims37-49, wherein the level of an autoantibody in the blood sample of thehuman subject is 5 times higher than the level of said autoantibodyrepresentative for a human subject of a healthy population.
 40. Themethod according to any of claims 28-35, 37-39, wherein the treatment iserlotinib.